基于双靶向黑色素纳米粒的肿瘤深穿透光声成像  

Deeply penetrating photoacoustic imaging in tumor tissues based on dual⁃targeted melanin nanoparticle

作　　者：曲波涛 王倩 宁晓刚 周雨欣 张瑞平[4] QU Botao;WANG Qian;NING Xiaogang;ZHOU Yuxin;ZHANG Ruiping(School of Basic Medical Sciences,Shanxi Medical University,Taiyuan 030001,China;School of Forensic Medicine,Shanxi Medical University,Taiyuan 030001,China;School of Chinese Medicine and Food Engineering,Shanxi University of Chinese Medicine,Jinzhong,Shanxi 030619,China;The Radiology Department of First Hospital of Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学基础医学院,太原030001 [2]山西医科大学法医学院,太原030001 [3]山西中医药大学中药与食品工程学院,晋中030619 [4]山西医科大学第一医院放疗科,太原030001

出　　处：《无机化学学报》2024年第5期1025-1032,共8页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.82120108016,82071987);国家万人计划项目(No.SQ2022RA2A300118)资助。

摘　　要：通过1-(3-二甲基氨基丙基)-3-乙基碳二亚胺和N-羟基丁二酰亚胺反应将透明质酸(hyaluronic acid,HA)和4-羧丁基三苯基溴化膦((4-carboxybutyl)triphenylphosphonium bromide,TPP)分子连接到聚乙二醇-氨基(PEG-NH_(2))修饰过的黑色素纳米颗粒(melanin nanoparticles,MNP)表面,设计合成了一种具有双靶向能力的黑色素纳米颗粒(MNP-TPP-HA),赋予了黑色素的双靶向能力。体外三维(3D)多细胞肿瘤球荧光成像和活体肿瘤光声成像实验表明MNP-TPP-HA具有优异的肿瘤靶向穿透能力。A dual‐targeting melanin nanoparticle(MNP‐TPP‐HA)was constructed by grafting the carboxyl groups of hyaluronic acid(HA)and(4‐carboxybutyl)triphenylphosphonium bromide(TPP)onto the surface of polyethylene glycol‐amino(PEG‐NH_(2))modified MNP based on 1‐(3‐dimethylaminopropyl)‐3‐ethylcarbodiimide hydrochloride(EDC)and N‐hydroxy succinimide(NHS)reaction,endowing melanin with the ability of dual active targeting.The fluorescence imaging of in vitro three‐dimensional(3D)tumor models and in vivo photoacoustic imaging(PAI)all reveal the excellent target penetration ability of MNP‐TPP‐HA.

关 键 词：双靶向 黑色素 光声成像 穿透能力 

分 类 号：O641.4[理学—物理化学] R730.5[理学—化学]