氮浓度和光照对嗜碳杜氏盐藻HTBS生长的影响  

Effects of Nitrogen Concentration and Light Intensity on the Growth of Carbophilic Dunaliella salina HTBS

作　　者：罗晨 郭之乐 姚沅江 路思晗 侯余勇[2] 王巍杰 LUO Chen;GUO Zhile;YAO Yuanjiang;LU Sihan;HOU Yuyong;WANG Weijie(School of Life Sciences,North China University of Science and Technology,Tangshan,Hebei 063210,China;Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China)

机构地区：[1]华北理工大学生命科学学院,河北唐山063210 [2]中国科学院天津工业生物技术研究所,天津300308

出　　处：《天津农业科学》2024年第4期1-6,共6页Tianjin Agricultural Sciences

基　　金：河北省大学生创新创业训练计划项目(X2022360)。

摘　　要：为探究不同氮浓度和光照强度对嗜碳杜氏盐藻HTBS生长的影响,通过设置培养基中不同的硝酸钠浓度(0、7.5、75、750、500 mg·L^(-1))和光照强度(20、80、140μmol·m^(-2)·s^(-1))对HTBS培养过程中细胞密度、生物量、色素含量、荧光参数进行研究。结果表明:750 mg·L^(-1)硝酸钠能够有效促进HTBS生长,对HTBS光合系统促进作用效果最明显,细胞密度和生物量增长最大,分别增长62.6%和33.4%;叶绿素含量增长44.5%;β-胡萝卜素降低19%;Qp增加,NPQ下降,Fv/Fm提升44.59%,ΦPSII提升125.00%。光照强度为80μmol·m^(-2)·s^(-1)和20μmol·m^(-2)·s^(-1)光照强度条件成长的HTBS相比,细胞密度和生物量分别增长21.5%和19.6%,藻体细胞叶绿素含量、β-胡萝卜素含量分别降低28.3%和19%,rETR值增加165.12%,Fv/Fm值、ΦPSII、NPQ、Qp呈下降趋势,化合效率降低。总之,光照强度为80μmol·m^(-2)·s^(-1)、硝酸钠为750 mg·L^(-1)时,能促进嗜碳杜氏盐藻HTBS细胞密度和生物量的增长。To investigate the effects of different nitrogen concentrations and light intensities on the growth of high bicarbonate tolerance strain Dunaliella salina HTBS,the cell density,biomass,pigment content,and fluorescence parameters during HTBS cultivation by varying nitrate concentrations(0 mg·L^(-1),7.5 mg·L^(-1),75 mg·L^(-1),750 mg·L^(-1),500 mg·L^(-1))and light intensities(20μmol·m^(-2)·s^(-1),80μmol·m^(-2)·s^(-1),140μmol·m^(-2)·s^(-1))were examined.The results showed that 750 mg·L^(-1) sodium nitrate could effectively promote the growth of HTBS with the most pronounced effect on the HTBS photosynthetic system.The most significant increases were observed in cell density and biomass growth,with increases of 62.6%and 33.4%respectively.The chlorophyll content increased by 44.5%,while theβ-carotene content decreased by 19%.Qp increased,while NPQ decreased.Furthermore,Fv/Fm increased by 44.59%,andΦPSII increased by 125.00%.In comparison to 20μmol·m^(-2)·s^(-1),80μmol·m^(-2)·s^(-1) increased cell density and biomass by 21.5%and 19.6%respectively,while the chlorophyll content andβ-carotene content decreased by 28.3%and 19%respectively.The rETR value exhibited a 165.12%increase,while the Fv/Fm value,ΦPSII,NPQ and Qp demonstrated a downward trend,accompanied by a decline in chemical efficiency.In conclusion,it could be concluded that a light intensity of 80μmol·m^(-2)·s^(-1) and a nitrogen concentration of 750 mg L^(-1) of sodium nitrate were optimal for the growth of the high bicarbonate tolerance microalga strain Dunaliella salina HTBS.

关 键 词：杜氏盐藻 嗜碳藻 氮源 光照强度 

分 类 号：Q89[生物学]