尼可地尔对大鼠颈总动脉球囊拉伤模型损伤血管再内皮化的影响  

Effects of Nicorandil on Reendothelialization of Injured Artery from Animal Model of Balloon Injury in Rat Carotid Artery

作　　者：王宁远 赖图雅 王鑫焱 李慧颖 王威 WANG Ningyuan;LAI Tuya;WANG Xinyan;LI Huiying;WANG Wei(Department of Cardiology,the Sixth Medical Center,Chinese PLA General Hospital,Beijing 100048,China;Department of Cardiology,Uxin Banner General Hospital of Mongolian Medicine,Ordos 017000,China;Unit 93658 of Chinese PLA,Beijing 100043,China;Department of Cardiology,the Second Medical Center,Chinese PLA General Hospital,Beijing 100094,China;Chinese PLA Medical School,Beijing 100853,China)

机构地区：[1]中国人民解放军总医院第六医学中心心血管病医学部,北京100048 [2]内蒙古自治区鄂尔多斯市乌审旗蒙医综合医院心脏病科,鄂尔多斯017000 [3]中国人民解放军93658部队,北京100043 [4]中国人民解放军总医院第二医学中心心血管内科,北京100094 [5]中国人民解放军医学院,北京100853

出　　处：《实验动物科学》2024年第2期1-5,共5页Laboratory Animal Science

基　　金：国家自然科学基金(81970443);中国人民解放军总医院第六医学中心创新培育基金(CXPY202202)。

摘　　要：目的研究尼可地尔对大鼠颈总动脉球囊拉伤模型损伤血管再内皮化的影响。方法将18只Sprague-Dawley(SD)大鼠随机分为3组,分别为假手术组、对照组、实验组,每组6只,对照组和实验组行颈总动脉球囊拉伤,假手术组仅分离颈总动脉,术后对照组给予0.9%氯化钠溶液(0.5 mL/100 g),每日1次;实验组和假手术组给予尼可地尔(2 mg/100 g)灌胃,每日1次,连续14 d,取大鼠颈总动脉血管标本。伊文氏蓝染色观察各组血管内膜损伤面积及血管再内皮化的情况,HE染色观察各组血管内膜增生及管腔狭窄程度。结果假手术组血管内膜光滑,内皮层完整,血管内膜未见增生。对照组球囊损伤14 d后内膜增生明显,新生内膜面积显著高于假手术组,差异具有统计学意义(P<0.05)。实验组球囊损伤14 d后新生内膜面积显著低于对照组,损伤血管再内皮化区域占比显著高于对照组,差异具有统计学意义(P<0.05)。结论尼可地尔可能抑制大鼠颈总动脉球囊拉伤后的内膜增生,促进损伤血管再内皮化。Objective To investigate the effect of nicorandil on reendothelialization of injured artery from rat carotid artery balloon-injured model.Method A total of 18 Sprague-Dawley(SD)rats were randomly divided into 3 groups:sham operation group,control group and experimental group,with 6 rats in each group.The common carotid artery was deendothelialised in the control group and the experimental group,while only the common carotid artery was isolated in the sham operation group.After operation,the control group was given 0.9%sodium chloride solution(0.5 mL/100 g,once a day),the experimental group and sham operation group were given nicorandil(2 mg/100 g,once a day)by intragastric administration for 14 days,and common carotid artery samples were collected.The lesion area and reendothelialisation of the vascular intima were observed by Ivans’blue staining,and the intima hyperplasia and lumen stenosis were observed by hematoxylin-eosin(HE)staining.Result In the sham operation group,the vascular intima was smooth,the endothelium was intact,and there was no hyperplasia in the vascular intima.After 14 days of balloon injury,endothelial hyperplasia was obvious in control group,and the area of neointimal was significantly higher than that in sham operation group,the difference was statistically significant(P<0.05).After 14 days of balloon injury,the area of neointimal in the experimental group was significantly lower than that in the control group,and the proportion of reendothelialization area in the injured arteries was significantly higher than that in the control group,with statistical significance(P<0.05).Conclusion The effects by which nicorandil can facilitate reendothelialization and attenuate intimal hyperplasia of animal model of balloon injury in rat carotid artery are marked.

关 键 词：再内皮化 尼可地尔 新生内膜增生 

分 类 号：Q95-3[生物学—动物学]