三种动物麻醉剂对C57BL/6J小鼠麻醉效果及血清生化指标的影响  

Effect of Three Animal Anesthetics on Anesthetic Efficacy and Serum Biochemical Indicators in C57BL/6J Mice

作　　者：刘军[1] 丁登峰 高伟[1] 贾云晓 赵常琦 牛苗苗 LIU Jun;DING Dengfeng;GAO Wei;JIA Yunxiao;ZHAO Changqi;NIU Miaomiao(Medical Innovation Research Division of Chinese PLA General Hospital,Beijing 100853,China)

机构地区：[1]中国人民解放军总医院医学创新研究部实验动物中心,北京100853

出　　处：《实验动物科学》2024年第2期51-58,共8页Laboratory Animal Science

基　　金：军队实验动物专项科研课题(SYDW[2020]01,SYDW[2020]02)。

摘　　要：目的比较三种动物麻醉剂三溴乙醇、戊巴比妥钠、舒泰50联合盐酸塞拉嗪的麻醉效果及对小鼠血清生化指标的影响,探索三种麻醉剂麻醉C57BL/6J(B6)小鼠的合适剂量范围。方法采用健康SPF级雄性B6小鼠100只随机分为10组,每组10只,三种麻醉剂分别以高、中、低剂量梯度对B6小鼠进行腹腔注射,比较三种麻醉剂不同剂量的麻醉诱导时间、维持时间、恢复时间及对B6小鼠血清ALT、AST、GLU和UA指标的影响。结果三种麻醉剂的麻醉维持时间和恢复时间与剂量呈正比,诱导时间与剂量呈反比。中(250 mg/kg)低(125 mg/kg)剂量三溴乙醇(1.25%)、中(80 mg/kg)低(40 mg/kg)剂量戊巴比妥钠(1.0%)及中(60 mg/kg)低(30 mg/kg)剂量舒泰50(0.50%)联合盐酸塞拉嗪(0.50%,中剂量12 mg/kg、低剂量6 mg/kg)对B6小鼠的麻醉相关时间较为适中,对肝肾功能影响较小。结论三种麻醉剂的不同剂量对B6小鼠的麻醉时间效果和血清生化指标的影响不尽相同;三溴乙醇在125~250 mg/kg范围内麻醉效果较好;戊巴比妥钠在40~80 mg/kg范围内麻醉效果较好;舒泰50联合盐酸塞拉嗪在30~60 mg/kg(盐酸塞拉嗪6~12 mg/kg)范围内麻醉效果较好;在涉及ALT、AST、GLU和UA的研究过程中,需根据不同实验需求选择合适剂量的麻醉剂。Objective To compare the anesthetic effects of three animal anesthetics:tribromoethanol,pentobarbital Sodium,zoletil 50 combined with serazine hydrochloride and their effects on serum biochemical indicators in mice.To explore the suitable dose range of three anesthetics for anesthesia of B6 mice.Method 100 healthy SPF grade male B6 mice were randomly divided into 10 groups,with 10 mice in each group.Three types of anesthetics were administered intraperitoneally to B6 mice at high,medium,and low dose gradients,respectively.Compared the induction time,maintenance time,recovery time,and effects on serum ALT、AST、GLU and UA indicators of B6 mice under different doses of three anesthetics.Result The anesthesia maintenance time and recovery time of the three anesthetics were proportional to the dose,while the induction time was inversely proportional to the dose.Medium(250 mg/kg)low(125 mg/kg)doses of tribromoethanol(1.25%),medium(80 mg/kg)low(40 mg/kg)doses of pentobarbital sodium(1.0%),and medium(60 mg/kg)low(30 mg/kg)doses of Zoletil 50(0.50%)combined with serazine hydrochloride(0.50%,medium dose 12 mg/kg,low dose 6 mg/kg)had moderate anesthesia related time and little impact on liver and kidney function in B6 mice.Conclusion The anesthetic effects and serum biochemical indicators of the three anesthetics on B6 mice were different.Tribromoethanol had a suitable anesthesia effect in the range of 125-250 mg/kg;Pentobarbital sodium had a suitable anesthetic effect in the range of 40-80 mg/kg;Zoletil 50 combined with serazine hydrochloride had a suitable anesthesia effect within the range of 30-60 mg/kg(Serazine Hydrochloride 6-12 mg/kg).In studies involving ALT,AST,GLU,and UA,appropriate doses of anesthetics need to be selected based on different experimental needs.

关 键 词：麻醉剂 小鼠 三溴乙醇 戊巴比妥钠 舒泰 盐酸塞拉嗪 生化指标 

分 类 号：Q95-3[生物学—动物学]