不同固定液对光镜下金黄地鼠海马体神经元的影响  

Effects of Different Fixing Solution on Hippocampal Neurons of Golden Hamster under Light Microscopee

作　　者：高明 张楠 李怀银 金娟 谢澎 GAO Ming;ZHANG Nan;LI Huaiyin;JIN Juan;XIE Peng(Experimental Animal Department of Lanzhou Institute of Biological Products Co.,Ltd.,Center for Gansu Provincial Vaccine Engineering Research,Lanzhou 730046,China)

机构地区：[1]兰州生物制品研究所有限责任公司实验动物室甘肃省疫苗工程技术研究中心,兰州730046

出　　处：《实验动物科学》2024年第2期59-65,共7页Laboratory Animal Science

摘　　要：目的比较不同固定液对光镜下金黄地鼠海马体神经元的影响,为金黄地鼠海马体神经元固定液的选择提供参考。方法将21只11周龄雄性SPF级金黄地鼠随机分为7组,分别使用4%多聚甲醛、10%中性甲醛、Bouin’s、Carnoy、Davidson’s、Zenker、Helly溶液进行心脏灌注,剥离脑壳取出完整大脑,在对应的固定液中固定48 h,并制作成石蜡切片,经HE染色后在光镜下观察海马体神经元的形态特征。通过匈牙利3DHISTECH公司数字切片扫描系统测量尺测量神经元细胞直径,比较固定液对细胞收缩的影响。结果4%多聚甲醛、10%中性甲醛、Bouin’s溶液展现了良好的固定效果,固定后的大脑硬度适中,HE染色后的切片着色适中、色泽鲜艳、神经元各种细胞形态对比清晰,4%多聚甲醛因固定后切片无龟裂而更为理想。不同固定液固定后的海马体神经元存在细胞收缩方面的极显著差异(P<0.01),使用Carnoy、Davidson’s固定后的神经元各种细胞收缩最严重。结论4%多聚甲醛、10%中性甲醛、Bouin’s溶液是金黄地鼠海马体神经元较为理想的固定液,4%多聚甲醛溶液建议作为首选。Objective To compare the effects of different fixations on neurons in hippocampus of golden hamster under light microscope,and to provide reference for the selection of fixations in hippocampus of golden hamster.Method 2111-week-old male SPF golden hamsters were randomly divided into 7 groups.The heart was injected with 4%paraformaldehyde,10%neutral formaldehyde,Bouins,Carnoy,Davidsons,Zenker and Helly solution,and the whole brain was removed.It was fixed in the corresponding fixing solution for 48 h,and made into paraffin sections.After HE staining,the morphological characteristics of hippocampus neurons were observed under the light microscope.The diameter of neuronal cells was measured by the measuring scale of 3DHISTECH digital slice scanning system in Hungary,and the effect of fixation solution on cell contraction was compared.Result 4%paraformaldehyde,10%neutral formaldehyde,Bouins solution showed a good fixing effect,the fixed brain hardness is moderate,HE stained slices with moderate coloring,bright color,neuron cell morphology contrast is clear,4%paraformaldehyde is more ideal because the slices without cracking after fixation.Hippocampal neurons immobilized with different fixations showed significant differences in cell shrinkage(P<0.01),and the cell shrinkage of neurons immobilized with Carnoy and Davidsons was the most severe.Conclusion 4%paraformaldehyde,10%neutral formaldehyde and Bouins solution are ideal fixation fluids for hippocampus neurons of golden hammers,and 4%paraformaldehyde solution is recommended as the first choice.

关 键 词：固定液 金黄地鼠 海马神经元 石蜡切片 

分 类 号：Q95-3[生物学—动物学]