功能性便秘大鼠造模的文献与实验研究  

Literature and Experimental Study on the Establishment of Functional Constipation Model in Rats

作　　者：熊静 王福民 滕雨可 郭雨怡 曾芳[1] 何昭璇 XIONG Jing;WANG Fumin;TENG Yuke;GUO Yuyi;ZENG Fang;HE Zhaoxuan(College of Acu-moxibustion and Tuina,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;West China Tianfu Hospital of Sichuan University,Chengdu 610021,China)

机构地区：[1]成都中医药大学针灸推拿学院,成都610075 [2]四川大学华西天府医院,成都610021

出　　处：《实验动物科学》2024年第2期78-84,共7页Laboratory Animal Science

基　　金：国家自然科学基金青年科学基金(81804002);成都中医药大学“杏林学者”学科人才科研提升计划(QNXZ2020008);四川省自然科学基金项目青年基金(2023NSFSC1820)。

摘　　要：目的基于文献分析结果进行功能性便秘(FC)大鼠造模方法的探索,以期为今后便秘相关动物实验提供参考。方法实验分为两部分:(1)将28只大鼠随机分为空白组(8只)、复方地芬诺酯组(10只)、盐酸洛哌丁胺组(10只),空白组灌胃0.9%氯化钠溶液,实验组分别采用灌胃10 mg/kg复方地芬诺酯混悬液、盐酸洛哌丁胺混悬液两种方法复制FC大鼠模型,14 d后观察3组大鼠首粒黑便排出时间、24 h排便数及粪便含水率等一般指标的差异。(2)30只大鼠随机分为空白组、小剂量组、大剂量组,每组各10只,实验组灌胃不同剂量的复方地芬诺酯混悬液,14 d后观察相关胃肠动力指标。结果实验一结果显示,3组大鼠的一般指标差异均无统计学意义(P>0.05);实验二结果显示,与空白组相比,大剂量组首粒黑便排出时间延长,粪便含水率、24 h排便数、小肠推进率、胃排空率降低(P<0.05);与小剂量组相比,大剂量组大鼠的首粒黑便排出时间较长,粪便含水率、24 h排便数、小肠推进率、胃排空率降低,其中24 h排便数、胃排空率差异有统计学意义(P<0.05)。结论10、15 mg/kg复方地芬诺酯及10 mg/kg盐酸洛哌丁胺制作FC模型失败;20 mg/kg复方地芬诺酯可成功复制FC模型。Objective Based on the result of the literature analysis,we explored the establishment of FC model in rats to provide areference for future animal experiments related to constipation.Method The experiment was divided into two parts.Firstly,28 rats were randomly divided into blank group(8 rats),compound diphenoxylate group(10 rats)and loperamide group(10 rats).The blank group was given 0.9%sodium chloride solution.The experimental groups were given 10 mg/kg compound diphenoxylate or loperamide to establish FC rat model.After 14 days,the differences of the first black stool discharge time,the number of 24 h defecation and fecal water content of the three groups were observed.In the second experiment,thirty rats were randomly divided into blank group,low-dose group and high-dose group.The experimental groups were given different doses of compound diphenoxylate by gavage,and the related gastrointestinal motility indexes were observed after 14 days.Result The result of the first experiment showed that there was no significant difference in the indexes among the three groups(P>0.05);the result of the second experiment showed that,compared with the blank group,the time of the first black stool discharge was prolonged in the high-dose group,and the fecal water content,the number of 24 h defecation,the rate of small intestinal propulsion,and the rate of gastric emptying were decreased(P<0.05);compared with the small-dose group,the time of the first black stool discharge was prolonged in the high-dose group,and the fecal water content,24 h defecation number,small intestinal propulsion rate,and gastric emptying rate decreased,among which the differences in 24 h defecation number and gastric emptying rate were statistically significant(P<0.05).Conclusion Oral administration of 10 mg/kg and 15 mg/kg compound diphenoxylate and 10 mg/kg loperamide failed to establish FC model.20 mg/kg compound diphenoxylate could establish FC model successfully.

关 键 词：便秘模型 复方地芬诺酯 盐酸洛哌丁胺 文献研究 实验 

分 类 号：Q95-3[生物学—动物学]