复方加味公英散在小鼠急性毒性试验中的应用  

Application Effect of Modified Gongying Powder Compound in Acute Toxicity Test of Mice

作　　者：秦美慧 田耕 郭嘉栋 万金隆 陶大勇[1] QIN Meihui;TIAN Geng;GUO Jiadong;WAN Jinong;TAO Dayong(College of Animal Science and Technology,Tarim University,Alar Xinjiang 843300,China)

机构地区：[1]塔里木大学动物科学与技术学院,新疆阿拉尔843300

出　　处：《现代畜牧科技》2024年第5期1-3,共3页Modern Animal Husbandry Science & Technology

摘　　要：通过小鼠急性毒性试验评价治疗奶牛乳房水肿病中药复方加味公英散的安全性,将复方制备成相当于原药材1.0 g/mL的水煎液,选取40只小鼠,随机分为高剂量组、中剂量组、低剂量组、对照组,以40、20、10 g/kg体重的剂量灌胃给药,对照组灌胃等体积的生理盐水,给药后连续观察7 d,记录死亡数和计算半数致死量(LD50);另取20只小鼠,随机分为2组,试验组以最大剂量(40 g/kg)、最大灌胃体积(40 mL/kg)灌胃小鼠,对照组灌胃等体积的生理盐水,给药后连续观察7 d,观察小鼠临床表现、体重变化及死亡情况。结果表明,急性毒性试验各剂量小鼠均无死亡,无明显毒性反应,无法计算LD50,判属无毒级别,小鼠体重变化、脏器系数与对照组相比无显著差异(P>0.05)。The safety of the traditional Chinese medicine compound modified gongying powder for the treatment of dairy cow breast edema was evaluated by acute toxicity test in mice.The Gongying powder was prepared into a water decoction equivalent to 1.0 g/mL of the original medicinal material.40 mice were randomly divided into high-dose group,medium-dose group,low-dose group and control group.The mice were given 40 g/kg,20 g/kg and 10 g/kg body weight by intragastric administration.The control group was given an equal volume of normal saline.After continuous observation for 7 days,the number of deaths was recorded and the median lethal dose(LD50)was calculated.Another 20 mice were randomly divided into two groups.The experimental group was intragastrically administered with the maximum concentration(40 g/kg)and the maximum intragastric volume(40 mL/kg),and the control group was intragastrically administered with the same volume of normal saline.After administration,the clinical manifestations,body weight changes and death of the mice were observed for 7 days.The results showed that there was no death in each dose of mice in the acute toxicity test,no obvious toxic reaction,and the LD50 could not be calculated,which was judged to be non-toxic.There was no significant difference in body weight change and organ coefficient between the mice and the control group(P>0.05).

关 键 词：加味公英散 奶牛 乳房水肿 急性毒性 

分 类 号：R285.1[医药卫生—中药学]