B群链球菌暴露后蜕膜间质细胞死亡和炎症信号的相关性研究  

Correlation between apoptosis of decidual interstitial cell and inflammatory signal following group B Streptococcus exposure

作　　者：张聪颖[1] 乔金凤[1] 金爽 陈丹 王海艳[1] 汪洁[1] 杨彩凤[1] ZHANG Congying;QIAO Jinfeng;JIN Shuang(Baoding No.2 Central Hospital,Hebei,Baoding 072750,China)

机构地区：[1]河北省保定市第二中心医院,072750

出　　处：《河北医药》2024年第10期1504-1507,1511,共5页Hebei Medical Journal

基　　金：保定市科学技术研究与发展计划项目(编号:2041ZF222)。

摘　　要：目的探究B群链球菌暴露后蜕膜间质细胞死亡和炎症信号的相关性。方法选取2017年10月至2019年10月于保定市第二中心医院妇产科分娩的孕妇100例,分为早产组27例和足月顺产组73例,取孕妇阴道拭子和蜕膜间质组织,检测B群链球菌阳性率、蜕膜间质细胞凋亡情况、血清炎性因子水平、NLRP3、Caspase-1、OX40、OX40L、PD-1和PD-L1 mRNA表达,分析B群链球菌暴露后蜕膜间质细胞死亡和炎症信号的相关性。结果2组孕妇B群链球菌阳性率、蜕膜间质细胞凋亡情况、血清炎性因子水平、NLRP3、Caspase-1、OX40、OX40L、PD-1和PD-L1 mRNA表达比较,差异均有统计学意义(P<0.05)。早产组孕妇的GBS阳性率和蜕膜组织细胞凋亡值分别为37.04%和(46.73±4.92),足月顺产组的GBS阳性率和蜕膜组织细胞凋亡值仅为9.59%和(6.03±2.04);早产组孕妇的血清炎性因子水平高于足月顺产组,蜕膜间质细胞凋亡的孕妇血清炎性因子水平高于未凋亡的孕妇,差异有统计学意义(P<0.05)。结论B群链球菌暴露后蜕膜间质细胞死亡和炎症信号存在相关性,蜕膜间质细胞死亡会引发炎性因子水平升高。Objective To investigate the correlation between apoptosis of decidual interstitial cell and inflammatory signal following group B Streptococcus(GBS)exposure.Methods One hundred pregnant women visited the Department of Gynaecology and Obstetrics of Baoding No.2 Central Hospital from October 2017 to October 2019 were divided into preterm delivery group(n=27)and full-term natural labor group(n=73).The vaginal swabs and decidual stromal tissues were collected for the detection for the positive rate of GBS,the apoptosis value of decidual stromal cells,the levels of serum inflammatory factors,the messenger RNA(mRNA)expression of NOD-like receptor protein 3(NLRP3)/Caspase-1,OX40/OX40L,and programmed cell death protein 1/programmed cell death ligand 1(PD-1/PD-L1)pathway.Moreover the correlation between apoptosis of decidual stromal cell and inflammation signal after GBS exposure was analyzed.Results There were significant differences in the mRNA expressions of NLRP3/caspase-1,OX40/OX40L,PD-1/PD-L1 pathway between groups(P<0.05).The positive rate of GBS(37.04%vs 9.59%)and the apoptosis value of decidual stromal cells(46.73±4.92 vs 6.03±2.04)in preterm delivery group were significant higher those of full-term natural labor groups(P<0.05).The levels of serum inflammatory factors in premature delivery group were significantly higher than those of full-term natural labor group,and which in the pregnant women with apoptosis of decidual interstitial cell were significantly higher than those without apoptosis of decidual interstitial cell(P<0.05).Conclusion There is a significant correlation between apoptosis of decidual interstitial cell and inflammatory signals following GBS exposure,and the apoptosis of decidual interstitial cell may result in a increase of inflammatory factors levels.

关 键 词：B群链球菌 蜕膜间质细胞 炎症信号 

分 类 号：R378.12[医药卫生—病原生物学]