基于非靶向代谢组学探究扁柏精油对赤拟谷盗的作用机制  

Exploring the mechanism of action of Chamaecyparis obtusa essential oil on Tribolium castaneum based on non-targeted metabolomics

作　　者：张莉睿 张宇 贺艳萍 戴煌 毕洁 ZHANG Lirui;ZHANG Yu;HE Yanping;DAI Huang;BI Jie(School of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China)

机构地区：[1]武汉轻工大学食品科学与工程学院,湖北武汉430023

出　　处：《河南工业大学学报（自然科学版）》2024年第2期114-122,共9页Journal of Henan University of Technology：Natural Science Edition

基　　金：国家自然科学基金项目(32102198)。

摘　　要：扁柏精油对赤拟谷盗具有较强控制作用,通过非靶向代谢组学技术从代谢层面探究其对赤拟谷盗的影响。利用LC-MS技术分析扁柏精油处理后的赤拟谷盗与对照组代谢物的差异。扁柏精油能够影响赤拟谷盗的代谢过程,共鉴定出262个差异代谢物(上调230个,下调32个),主要涉及氨基酸类相关化合物、萜类化合物、生物碱、苯丙酸类、聚酮化合物、脂肪酸相关化合物和黄酮类化合物。这些差异代谢物在35条KEGG代谢途径(包括氨基酸代谢、脂代谢、碳水化合物代谢、辅因子和维生素代谢、信号转导等)中显著富集。本研究为进一步认识扁柏精油对赤拟谷盗的潜在代谢影响提供了依据,并为开发更有效的昆虫控制策略奠定了基础。Tribolium castaneum(T.castaneum)is a pest that poses an important threat to food and grain production worldwide.In recent years,botanical insecticides have been widely promoted,in which plant essential oils are considered potential candidates for developing green insecticides.The previous bioassay studies of our research group showed that Chamaecyparis obtusa(C.obtuse)essential oil had certain contact activity against T.castaneum,and the LD 50 was 52.54μg/adult.At three concentrations(0.41,1.62,and 2.83 uL/cm 2),the repellent rates of C.obtusa essential oil against T.castaneum were all above 80%at 15,30,60,and 120 min,respectively,indicating that the repellent effect was strong.Meanwhile,the C.obtusa essential oil exhibited fumigant toxicity against T.castaneum with LC 50 values of 7.09μg/L air.In this study,we used LC-MS non-targeted metabolomics technology to find that C.obtusa essential oil could affect the metabolic profile of T.castaneum.A total of 262 differential metabolites were identified(up-regulated:230,down-regulated:32),mainly involving amino acid related compounds,terpenoids,alkaloids,phenylpropanoids,polyketides,fatty acids related compounds and flavonoids.The VIP value analysis results of the control group and the C.obtusa essential oil treatment group showed that the VIP values of the five metabolites of prenyl cis-caffeate,glutamylleucylarginine,(S)-abscisic acid,3-(8-hydroxyoctyl)phenol,and diethyl phthalate were higher,and the difference between the two samples was very significant.In addition,the KEGG pathway statistical analysis of the 262 differential metabolites screened showed that the differential metabolites in the control group and the essential oil treatment group were enriched into 35 pathways,involving 63 metabolites,mainly including amino acid metabolism,lipid metabolism,carbohydrate metabolism,cofactor and vitamin metabolism,signal transduction and other pathways.In summary,our results provide a basis for further understanding the potential metabolic effects of C.obtusa essentia

关 键 词：赤拟谷盗 精油 代谢组学 代谢物 通路分析 

分 类 号：S433.5[农业科学—农业昆虫与害虫防治]