基于香豆酮并色酮生物硫醇荧光探针的合成及应用研究  

Synthesis and application of fluorescent probes based on coumaronochromone biothiols

作　　者：杨政敏[1] 蒋庆科 胡先运[1] 覃江克 YANG Zheng-min;JIANC Qing-ke;HU Xian-yun;QIN Jiang-ke(Department of Pharmacy,Qiannan Medical College for Nationalities,Duyun 558003,China;School of Chemistry and Pharmaceutical Sciences,Guangxi Normal University,Guilin 541004,China)

机构地区：[1]黔南民族医学高等专科学校药学系,贵州都匀558003 [2]广西师范大学化学与药学学院,广西桂林541004

出　　处：《化学研究与应用》2024年第5期1048-1055,共8页Chemical Research and Application

基　　金：国家自然科学基金项目(81860317)资助;贵州省卫生健康委员会科学基金项目(zwkj2021-453)资助;黔南民族医学高等专科学校基金项目(Qnyz2021001,Qnyz2023001)资助。

摘　　要：为监测具有高度生物学活性的谷胱甘肽(GSH),半胱氨酸(Cys)和高半胱氨酸(Hcy)等生物硫醇的生物功能,基于丙烯酸酯对生物硫醇的识别,通过三步反应设计合成香豆酮并色酮的荧光探针JA检测生物硫醇。探针JA具有反应快(10 min响应Cys)、抗干扰性强(Stokes位移150 nm)、毒性低和生物相容性好等特点,能对活细胞内外源性的生物硫醇进行荧光成像。探针JA为生物硫醇的灵敏检测提供了一种简单有效的方法。To monitor the biological functions of highly biologically active biothiols such as glutathione(GSH),cysteine(Cys)and homocysteine(Hcy),based on the recognition of biothiols by acrylates,a three-step reaction was designed to synthesize the fluorescent probe JA for the detection of biothiols by coumaronochromone.The probe JA was characterized by fast response(10 min response to Cys),high immunity to interference(Stokes shift of 150 nm),low toxicity and biocompatibility,and was capable of fluorescent imaging of exogenous biothiols in living cells.Probe JA provided a simple and effective method for the sensitive detection of biothiols.

关 键 词：生物硫醇 香豆酮并色酮 荧光探针 荧光成像 

分 类 号：O482.31[理学—固体物理]