EDTA对重金属胁迫下匍匐翦股颖种子萌发的影响  

Effects of EDTA on seed germination of creeping bentgrass under heavy metal stress

作　　者：赵心笛 刘敏婷 刘容 李振华[1] 张馨馨 路佳音 柴琦[1] ZHAO Xin-di;LIU Min-ting;LIU Rong;LI Zheng-hua;ZHANG Xin-xin;LU Jia-yin;CHAI Qi(Key Laboratory of Grassland and Animal Husbandry Innovation of the Ministry of Agriculture and Rural Affairs of Lanzhou University,Engineering Research Center of Grassland Agriculture Ministry of Education of Lanzhou University/College of Pastoral Agriculture Science and Technology,Lanzhou 730020,China)

机构地区：[1]兰州大学农业农村部草牧业创新重点实验室,兰州大学草地农业教育部工程研究中心,草地农业科技学院,甘肃兰州730020

出　　处：《草原与草坪》2024年第2期67-78,共12页Grassland and Turf

基　　金：四川省甘孜州横向项目-CZ铁路生态脆弱地带高寒草甸恢复技术研究项目(CSCEC-2021-S-2)。

摘　　要：【目的】研究外源EDTA对重金属胁迫下匍匐翦股颖(Agrostis stolonifera)种子萌发的影响。【方法】以匍匐翦股颖品种潘威为试验材料,对不同浓度Cu、Cd、Pb胁迫下的匍匐剪股颖进行EDTA处理,并测定匍匐翦股颖种子发芽率、发芽势、发芽指数、幼苗叶片及根系相关指标。【结果】匍匐翦股颖对Pb的耐受能力最强,Cu次之,Cd最差。施加3 mmol/L EDTA对中、重度重金属胁迫下匍匐翦股颖的发芽指标、叶片指标和根系指标均较优,其中,中度Cu、轻度Cd、中度Pb胁迫下施用3 mmol/L EDTA的匍匐翦股颖种子的发芽率分别较对照增加了16.67%、3.36%、8.72%,叶片长度分别增加了30.99%、30.60%、1.63%,根系长度分别增加了35.93%、0.37%、29.80%。【结论】通过隶属函数法对各处理进行综合排序,发现施用3 mmol/L EDTA匍匐翦股颖对重金属胁迫的缓解效果最优,对3种重金属的缓解效果排序为Pb>Cu>Cd。【Objective】In order to study the effect of exogenous EDTA on seed germination of creeping bent⁃grass(Agrostis stolonifera L.)under heavy metal stress.【Method】The creeping bentgrass′Pennway′variety was used as the test material,and the creeping bentgrass under different concentrations of Cu,Cd and Pb stress was treated with EDTA,and the seed germination rate,germination potential,germination index,seedling leaf and root re⁃lated indexes of creeping bentgrass were determined.【Result】The results showed that creeping bentgrass had the strongest tolerance to Pb,followed by Cu and Cd.The application of 3 mmol/L EDTA had better germination index,leaf index and root index of creeping bentgrass under moderate and severe heavy metal stress.Among them,the ger⁃mination rate of creeping bentgrass seeds treated with 3 mmol/L EDTA under moderate Cu,mild Cd and moderate Pb stress increased by 16.67%,3.36%and 8.72%,respectively,compared with the controls.The leaf length in⁃creased by 30.99%,30.60%and 1.63%,respectively,and the root length increased by 35.93%,0.37%and 29.80%,respectively.【Conclusion】Through the comprehensive ranking of each treatment by the membership func⁃tion method,it was found that the application of 3 mmol/L EDTA had the best mitigation effect on heavy metal stress,and the mitigation effect on the three heavy metals was ranked as Pb>Cu>Cd.

关 键 词：EDTA 重金属胁迫 匍匐翦股颖 种子萌发 

分 类 号：S688.4[农业科学—观赏园艺]