东祁连山高寒灌丛草甸小叶金露梅根部内生真菌多样性对季节的响应  

Seasonal response of endophytic fungal diversity in roots of Potentilla parvifolia in alpine scrub meadow of eastern Qilian Mountains

作　　者：邓秀霞 曹文侠[1] 王世林 王文虎 刘婉婷 李文 DENG Xiu-xia;CAO Wen-xia;WANG Shi-lin;WANG Wen-hu;LIU Wan-ting;LI Wen(College of Grassland Science,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem,Ministry of Education,Grassland Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing Land Eco-system Sustainability,Lanzhou 730070,China;Qinghai Academy of Animal and Veterinary Sciences(Academy of Animal and Veterinary Sciences,Qinghai University),Xining 810016,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]青海省畜牧兽医科学院(青海大学畜牧兽医科学院),青海西宁810016

出　　处：《草原与草坪》2024年第2期88-95,105,共9页Grassland and Turf

基　　金：国家自然基金项目(32060269);草地农业生态系统国家重点实验室开放基金课题。

摘　　要：【目的】明确高寒地区灌木小叶金露梅(Potentilla parvifolia)根部可培养内生真菌的多样性和季节变化。【方法】以东祁连山的优势灌木小叶金露梅为试验材料,利用组织培养法分离根部的内生真菌,用形态鉴定和ITS分子测序相结合进行真菌鉴定并分析其季节多样性。【结果】春季、夏季和秋季在小叶金露梅根段组织中分离得到71株真菌纯培养物,归属于5纲5目5科6属8个分类单元;不同季节的小叶金露梅根部内生真菌种类和数量存在一定差异,从多样性指数来看,Shannon-Wiener指数和Simpson’s diversity指数表现为春季(1.57,0.75)>夏季(1.26,0.69)>秋季(1.08,0.62)。春季根部内生真菌多样性高于夏季和秋季,但优势属均为脉孢菌属(Neurospora)和曲霉属(Aspergillu);春季特有属为青霉属(Penicillium)和色串孢属(Torula);赭霉属(Ochroconis)为夏季特有属,葡萄孢属(Botrytis)为秋季特有属。【结论】不同季节小叶金露梅根部均分离得到了内生真菌,且内生真菌在小叶金露梅根部的定殖存在季节差异性,春季根部内生真菌多样性较高。【Objective】To determine the diversity and seasonal variation of culturable endophytic fungi in the roots of Potentilla parvifolia in alpine region.【Method】Potentilla parvifolia,a dominant shrub in the eastern Qilian Mountains was used as the test material.The endophytic fungi in root were isolated by tissue culture,and their sea⁃sonal diversity was analyzed by morphological identification combined with ITS molecular sequencing.【Result】71 pure fungi were isolated from the tissue of the golden dew rhizome in spring,summer and autumn,belonging to 8 taxa of 5 classes,5 orders,5 families,6 genera.There were some differences in the species and quantity of endophytic fungi in the roots of Potentilla parvifolia in different seasons.From the diversity index,Shannon⁃Wiener index and Simpson′s diversity index showed as spring(1.57,0.75)>summer(1.26,0.69)>autumn(1.08,0.62).The di⁃versity of endophytic fungi in the roots in spring was higher than that in summer and autumn,but the dominant genera were Neurospora and Aspergillus.The endemic genera in spring were Penicillium and Torula;Ochroconis was en⁃demic in summer,and Botrytis was endemic in autumn.【Conclusion】The endophytic fungi were isolated from the roots of Potentilla parvifolia in different seasons,and the endophytic fungi colonization in the roots of Potentilla par⁃vifolia had seasonal differences,The diversity of endophytic fungi in the roots in spring was higher.

关 键 词：小叶金露梅 内生真菌 分子鉴定 季节多样性 

分 类 号：S812[农业科学—草业科学]