基于与间苯二酚的亚硝化反应光度法测定水中亚硝酸根  

Determination of Nitrite in Water by Spectrophotometric Method based on Nitration Reaction with Resorcinol

作　　者：刘海军[1] 李晓惠[1] LIU Haijun;LI Xiaohui(School of Biomedical and Chemical Engineering,Liaoning Institute of Science and Technology,Benxi Liaoning 117004,China)

机构地区：[1]辽宁科技学院生物医药与化学工程学院,辽宁本溪117004

出　　处：《辽宁科技学院学报》2024年第2期5-7,74,共4页Journal of Liaoning Institute of Science and Technology

基　　金：辽宁省教育厅重点科研项目(JYTZD2023191);辽宁科技学院博士科研启动基金项目(2307B12).

摘　　要：为拓宽光度法测定水中亚硝酸根的线性范围,文章提出了一种基于与间苯二酚的亚硝化反应测定水中亚硝酸根的新方法。在盐酸介质中,亚硝酸根与间苯二酚发生亚硝化反应生成对亚硝基间苯二酚,对亚硝基间苯二酚在pH值为9.97的碱性介质中快速异构化成橙黄色的邻羟基对苯醌单肟,采用分光光度计测定其在351 nm处的吸光度。结果表明,亚硝酸根浓度在0.08 mg/L~1.40 mg/L范围内与吸光度呈良好的线性关系,方法的检出限为0.02 mg/L,用于河水和池塘水中亚硝酸根的测定,回收率在96.3%~102.0%之间,相对标准偏差小于2.35%,测定值与标准方法的参考值吻合。该方法为环境水中亚硝酸根的测定提供了新方法和新思路。A new method based on the nitration reaction with resorcinol was proposed to broaden the linear range of spectrophotometric determination of nitrite in water.In an hydrochloric acid medium,nitrite reacted with resorcinol to form 4-nitrosobenzene-1,3-diol that rapidly isomerized to orange-yellow 2-hydroxy-p-benzoquinone oxime in an alkaline medium with pH of 9.97.The absorbance of the resulting compound was measured at 351 nm using a spectrophotometer.It was shown that there exists a good linear correlation between the absorbance and nitrite concentration within the range of 0.08 mg/L to 1.40 mg/L,and the detection limit of the method is 0.02 mg/L.This method has been successfully applied for the determination of nitrite in river water and pond water with the recovery between 96.3%~102.0% and the relative standard deviation less than 2.35%.Also,the measured values are in well agreement with the reference values obtained from standard methods.This method provides a new approach and perspective for the measurement of nitrite in environmental water.

关 键 词：亚硝酸根 间苯二酚 亚硝化 分光光度法 测定 

分 类 号：X832[环境科学与工程—环境工程] O657.32[理学—分析化学]