16S核糖体RNA基因测序评估高原红细胞增多症患者血液菌群特征  

Characteristics of blood microbiota in high altitude polycythemia patients by 16S rRNA gene sequencing

作　　者：黄沛 黄河 谢慎威 吴玉[1] 钟志凤 董华平[1] 周思敏[1] 李鹏[1] 谢佳新 HUANG Pei;HUANG He;XIE Shenwei;WU Yu;ZHONG Zhifeng;DONG Huaping;ZHOU Simin;LI Peng;XIE Jiaxin(Department of High Altitude Operational Medicine,Faculty of High Altitude Military Medicine,Army Medical University(Third Military Medical University),Chongqing,400038;Department of Neurology,No.940 Hospital of Joint Logistics Support Force of PLA,Lanzhou,Gansu Province,730000;Department of Health Management,No.953 Hospital of PLA Army,Shigatse,Xizang Autonomous Region,857000,China)

机构地区：[1]陆军军医大学(第三军医大学)高原军事医学系高原作业医学教研室,重庆400038 [2]联勤保障部队第九四〇医院神经内科,兰州730000 [3]陆军第九五三医院健康管理科,西藏日喀则857000

出　　处：《陆军军医大学学报》2024年第10期1075-1082,共8页Journal of Army Medical University

基　　金：甘肃省自然科学基金(22JR11RA008);第九四〇医院院内课题(2022yxky005);甘肃省卫生健康行业科研项目(GSWSKY2022-47)。

摘　　要：目的探讨高原红细胞增多症(high altitude polycythemia,HAPC)患者血液菌群变化特征及其与HAPC发病风险的相关性。方法采用横断面研究设计方案,以2021年在西藏日喀则陆军第九五三医院健康管理科参加体检的41名HAPC患者(HAPC组)和41名高原健康人员(对照组)为研究对象。通过高通量测序技术对血液细菌16 S核糖体RNA(16S rRNA)基因的V3-V4可变区进行检测,比较分析HAPC组和对照组血液菌群组成情况和差异。结果2组人员均为汉族男性,年龄、体质量指数及高原移居时间等基线数据比较差异无统计学意义(P>0.05)。菌群多样性分析显示,HAPC组中反映血液菌群α-多样性的Simpson指数[(0.931±0.005)vs(0.907±0.008),P<0.05]、Goods Coverage指数[(0.998±0.001)vs(0.997±0.001),P<0.001]和Pielou指数[(0.597±0.011)vs(0.567±0.009),P<0.05]显著高于对照组。2组间β多样性差异有统计学意义(P<0.01)。菌群相对丰度分析显示,HAPC组血液嗜糖假单胞菌属(Pelomonas)[(0.046±0.004)vs(0.033±0.003),P<0.05]、固氮螺菌属(Azospirillum)[(0.046±0.006)vs(0.021±0.003),P<0.01]、食酸菌属(Acidovorax)[(0.032±0.003)vs(0.019±0.002),P<0.05]的相对丰度均显著高于对照组。特征性细菌LEfSe分析显示,HAPC组标志菌群是α-变形菌纲,对照组标志菌群是毛螺菌科。结论HAPC患者较高原健康人群的血液菌群在多样性、相对丰度及特征性菌类方面发生显著变化,这类变化可能与HAPC的发生发展密切关联。Objective To investigate the changes in blood microbiota in patients with high altitude polycythemia(HAPC)and the correlation with the risk of HAPC.Methods A cross-sectional trial was carried out among 41 HAPC patients(HAPC group)and 41 healthy plateau individuals(control group)who took physical examination in the Health Management Department of No.953 Hospital of PLA Army in 2021.High-throughput sequencing technology was used to detect the V3-V4 variable region of the 16S ribosomal RNA(16S rRNA)gene in the blood smaples,and the composition and difference of the blood microbiota were compared and analyzed between the 2 groups.Results All the participants were male and Han people,and there were no significant differences in baseline data such as age,body mass index and plateau migration time between the 2 groups(P>0.05).Theα-diversity of blood microbiota in the HAPC group,including the Simpson index(0.931±0.005 vs 0.907±0.008,P<0.05),Goods Coverage index(0.998±0.001 vs 0.997±0.001,P<0.001)and Pielou index(0.597±0.011 vs 0.567±0.009,P<0.05)were significantly higher than those in the control group.Meanwhile,obvious difference was observed in the β diversity between the 2 groups(P<0.01).The relative abundance analysis of bacteria showed that Pelomonas(0.046±0.004 vs 0.033±0.003,P<0.05),Azospirillum(0.046±0.006 vs 0.021±0.003,P<0.01),Acidovorax(0.032±0.003 vs 0.019±0.002),Azospirillum(0.046±0.006 vs 0.021±0.003,P<0.01)and Acidovorax(0.032±0.003 vs 0.019±0.002,P<0.05)were statistically higher in the HAPC group than the control group.LEfSe analysis showed that the characteristic blood microbiota of the HAPC group wereα-Proteobacteria,and those of control group were Trichospiridae.Conclusion Significant changes are found in diversity,relative abundance and characteristic bacteria of the blood microbiota between the HAPC patients and healthy people at the high altitude,which might be closely associated with the occurrence and development of HAPC.

关 键 词：高原红细胞增多症 血液菌群 移居者 

分 类 号：R195.4[医药卫生—卫生统计学] R446.119[医药卫生—卫生事业管理] R555.1[医药卫生—公共卫生与预防医学]