Determination of Benzo[a]pyrene in Edible Oil by High Performance Liquid Chromatography-Fluorescence Detector (HPLC-FL)  

作　　者：Guixia YANG Jie LIU Xiujuan WANG Fenglan ZHANG Kun XIN Chunli KONG 

机构地区：[1]Jiaxiang Hengcheng Inspection Detection Co.,Ltd.,Jining 272400,China

出　　处：《Agricultural Biotechnology》2024年第2期8-9,19,共3页农业生物技术（英文版）

摘　　要：In this study, an optimized high performance liquid chromatography-fluorescence detector (HPLC-FL) method for the determination of benzo[a]pyrene in edible oil was established. HPLC was performed with Thermo Fisher Scientific C18 column (250 mm×4.6 mm, 5 μm) as the chromatographic column and acetonitrile and water as the mobile phase, and the excitation wavelength and emission wavelength of fluorescence detector were 286 and 430 nm, respectively. The response was high, and the linear range was 0.5-10.0 ng/ml. The lowest limit of detection was 0.11 ng/ml, and the average recovery was 92.5%. This method is suitable for quantitative analysis of benzo[a]pyrene content in edible oil.

关 键 词：BENZO[A]PYRENE High performance liquid chromatography Fluorescence detector 

分 类 号：O657.72[理学—分析化学] TS262.3[理学—化学]