基于生物信息学方法筛选并验证心肌细胞增殖及心肌再生的相关枢纽基因  

Identificaton and validation of hub genes related to cardiomyocyte proliferation and myocardial regeneration based on bioinformatics approaches

作　　者：杜小宇 汪澈 郑汝杰 刘旨浩 卢成志[3] DU Xiaoyu;WANG Che;ZHENG Rujie;LIU Zhihao;LU Chengzhi(The First Central Clinical School,Tianjin Medical University,Tianjin 300192,China;Department of Clinical Medicine,School of Medicine,Nankai University,Tianjin 300071,China;Department of Cardiology,Tianjin First Center Hospital,Tianjin 300192,China)

机构地区：[1]天津医科大学一中心临床学院,天津300192 [2]南开大学医学院临床医学系,天津300071 [3]天津市第一中心医院心内科,天津300192

出　　处：《天津医科大学学报》2024年第3期224-231,共8页Journal of Tianjin Medical University

基　　金：国家自然科学基金(81970303);天津市医学重点学科(专科)建设项目(TJYXZDXK-054B);天津市自然科学基金资助项目(21JCYBJC00250)。

摘　　要：目的:通过生物信息学筛选与心肌梗死(MI)后心肌细胞增殖与再生的相关枢纽基因。方法:从GEO数据库下载并合并数据集GSE123863、GSE184792和GSE198300,通过对样本进行加权基因共表达网络分析(WGCNA)筛选枢纽基因。并采用1日龄(增殖组)和7日龄(非增殖组)C57BL/6J乳鼠构建MI模型,每组6只,于术后3 d取MI区组织行实时荧光定量PCR(RT-qPCR)验证枢纽基因的表达情况。结果:通过生物信息学分析共筛选出7个枢纽基因:NDUFB2、CPEB4、TECR、HIC2、IFITM3、ACOT1和ACOT4。动物实验结果显示,与非增殖组相比,在增殖组中NDUFB2、TECR表达显著下降,CPEB4、HIC2、IFITM3和ACOT1表达显著上升(均P<0.05);ACOT4表达呈上升趋势,但无统计学意义(P>0.05)。结论:NDUFB2、CPEB4、TECR、HIC2、IFITM3和ACOT1可能在MI后心肌细胞增殖过程中发挥重要作用。Objective:To screen hub genes associated with cardiomyocyte proliferation after myocardial infarction(MI)by bioinformatics analysis.Methods:Datasets GSE123863,GSE184792,and GSE198300 were downloaded from the GEO database and merged.Weighted gene co-expression network analysis(WGCNA)was performed for samples to screen out the hub genes.Then MI model was constructed using 1-day-old(proliferating group)and 7-day-old(non-proliferating group)C57BL/6J neonatal mice,and 6 mice per group.The MI area was harvested at 3 days postoperatively for real-time quantitative PCR(RT-qPCR)to verify the expression of the hub gene.Results:A total of seven hub genes were screened by bioinformatics analysis:NDUFB2,CPEB4,TECR,HIC2,IFITM3,ACOT1,ACOT4.The results of animal experiments showed that compared with the non-proliferative group,the expression of NDUFB2 and TECR decreased significantly in the proliferative group,while the expression of CPEB4,HIC2,IFITM3,and ACOT1 increased significantly(all P<0.05).Meanwhile,the expression of ACOT4 showed a tendency of increase,but the difference was not statistically significant(P>0.05).Conclusion:NDUFB2,CPEB4,TECR,HIC2,IFITM3,and ACOT1 may play important roles in the proliferation of cardiomyocyte proliferation after MI.

关 键 词：心肌细胞增殖 心肌再生 心肌梗死 生物信息学 

分 类 号：R541.4[医药卫生—心血管疾病]