猴痘病毒新型蛋白疫苗制备及其诱导抗体能力评估  

Construction of a novel protein vaccine against monkeypox virus and evaluation of its immune response

作　　者：赵成燕 周兵 闫虎 李文婷 孙岳宏 鞠斌 张政 鲁晓擘[1] ZHAO Chengyan;ZHOU Bing;YAN Hu;LI Wenting;SUN Yuehong;JU Bin;ZHANG Zheng;LU Xiaobo(Infectious Disease and Liver Disease Department,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China;the Institute of Liver Disease,the Third People's Hospital of Shenzhen,Shenzhen Guangdong 518112,China)

机构地区：[1]新疆医科大学第一附属医院感染·肝病中心,乌鲁木齐830054 [2]深圳市第三人民医院肝病研究所,广东深圳518112

出　　处：《新疆医科大学学报》2024年第5期647-652,共6页Journal of Xinjiang Medical University

基　　金：国家自然科学基金项目(82060115,82025022)。

摘　　要：目的构建有效的猴痘病毒(MPXV)和痘苗病毒(VACV)蛋白疫苗,并对其诱导抗体的活性进行初步评估。方法采用融合蛋白表达技术构建MPXV-A29-Fc和VACV-A27-Fc蛋白疫苗,酶联免疫吸附试验(ELISA)和蛋白质印迹法(Western blot,WB)体外检测蛋白结合抗体的能力,BALB/c雌性小鼠体内评估不同剂量蛋白疫苗诱导抗体的效价。结果MPXV-A29-Fc和VACV-A27-Fc蛋白均能与A29单克隆抗体结合,蛋白疫苗免疫小鼠后,不同剂量组中的小鼠血清均可交叉识别MPXV-A29和VACV-A27蛋白,三针免疫可诱导更强的交叉免疫应答,三针免疫后,不同的血清稀释度下,低剂量组的IgG抗体结合强度均与高剂量组相当,可达到高剂量组的免疫效果。结论本研究初步构建有效的MPXV和VACV新型蛋白疫苗MPXV-A29-Fc和VACV-A27-Fc,在小鼠体内可诱导特异性抗体反应和交叉抗体反应,可为猴痘病毒的疫苗设计提供新的思路和方法。Objective To construct monkeypox virus(MPXV)and vaccinia virus(VACV)protein vaccines and evaluate antibody activity induced by them.Methods MPXV-A29-Fc and VACV-A27-Fc protein vaccines were constructed by fusion protein expression technology.The binding capacity of proteins was detected by ELISA and WB in vitro.Titer of antibody elicited by the protein was detected by immunizing female BALB/c mice in vivo.Results Both MPXV-A29-Fc and VACV-A27-Fc proteins could bind to A29 mAb.MPXV-A29 and VACV-A27 proteins can be cross-recognized by the serum of mice immunized with MPXV-A29-Fc and VACV-A27-Fc proteins.3-dose vaccination could induce stronger cross-immune responses.After 3 doses of MPXV-A29-Fc or VACV-A27-Fc in mice,the binding capacity of IgG antibody in the low-dose group was similar to that in the high-dose group at different serum dilutions,which could achieve the effective vaccination of the high-dose group.Conclusions In this study,novel vaccines MPXVA29-Fc and VACV-A27-Fc,which can induce specific antibody response and cross-antibody response in mice,and they are successfully prepared.It provides a new idea and candidate for the design of MPXV vaccine.

关 键 词：猴痘病毒(MPXV) 重组蛋白疫苗 结合活性 交叉反应性 

分 类 号：R373.9[医药卫生—病原生物学]