Guidelines for application of high-content screening in traditional Chinese medicine:concept,equipment,and troubleshooting  

作　　者：Xuechun Chen Lu Li Mingxu Zhang Jian Yang ChunMing Lyu Yizhou Xu Yang Yang Yi Wang 

机构地区：[1]Pharmaceutical Informatics Institute,College of Pharmaceutical Sciences,Zhejiang University,Hangzhou,China [2]Department of Cardiology,Hangzhou First People’s Hospital,Hangzhou,China [3]National Key Laboratory of Chinese Medicine Modernization,Innovation Center of Yangtze River Delta,Zhejiang University,Jiaxing,China [4]Molecular Devices Shanghai Corporation,Shanghai,China [5]National Key Laboratory of Chinese Medicine Modernization,Tianjin University of Traditional Chinese Medicine,Tianjin,China [6]Science and Technology Experiment Center,Shanghai University of Traditional Chinese Medicine,Shanghai,China [7]Engineering Research Center of Innovative Anticancer Drugs,Ministry of Education,hangzhou China

出　　处：《Acupuncture and Herbal Medicine》2024年第1期1-15,共15页针灸和草药（英文）

基　　金：supported by grants from the Construction Fund of Key Medical Disciplines of Hangzhou(OO20200121,China);“Pioneer”and“Leading Goose”R&D Program of Zhejiang(2023C03004,2024C03143).

摘　　要：High-content screening(HCS)technology combines automated high-speed imaging hardware and single-cell quantitative analysis.It can greatly accelerate data acquisition in cellular fluorescence imaging and is a powerful research technique in traditional Chinese medicine(TCM).An increasing number of laboratories and platforms,including TCM laboratories,have begun utilizing HCS systems.However,this technology is still in its infancy in TCM research and there is a lack of sufficient experience with the associated concepts,instrument configurations,and analysis methods.To improve the understanding of HCS among researchers in the field of TCM,this paper summarizes the concept of HCS,software and hardware configuration,the overall research process,as well as common problems and related solutions of HCS in TCM research based on our team’s previous research experience,providing several research examples and an outlook on future perspectives,aiming to provide a technical guide for HCS in TCM research.

关 键 词：High-content imaging High-content screening Traditional Chinese medicine 

分 类 号：R28[医药卫生—中药学]