西藏桑桑牦牛FGF1基因克隆及组织表达  

Cloning and Tissue Expression of FGF1 Gene from Tibetan Sangsang Yak

作　　者：王佟 余道宁 马超凡 张明昊 郑青波 陈伟基 马晓明 梁春年[2,3] 阎萍 潘和平[1] WANG Tong;YU Daoning;MA Chaofan;ZHANG Minghao;ZHENG Qingbo;CHEN Weiji;MA Xiaoming;LIANG Chunnian;YAN Ping;PAN Heping(College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030,China;Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Key Laboratory of Yak Breeding in Gansu Province,Lanzhou 730050,China;Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau,Ministry of Agriculture and Rural Affairs,Lanzhou 730050,China;Institute of Western Agriculture,Chinese Academy of Agricultural Sciences,Changji 831100,China)

机构地区：[1]西北民族大学生命科学与工程学院,兰州730030 [2]中国农业科学院兰州畜牧与兽药研究所,甘肃省牦牛繁育重点实验室,兰州730050 [3]农业农村部青藏高原畜禽遗传育种重点实验室,兰州730050 [4]中国农业科学院西部农业研究中心,昌吉831100

出　　处：《中国草食动物科学》2024年第3期1-9,共9页China Herbivore Science

基　　金：中央高校基本科研业务费专项(31920210160-01);西北民族大学动物遗传育种与繁殖创新团队(10015645);国家重点研发计划项目(2022YFD1302101);西藏自治区区域科技协同创新项目(QYXTZX-RKZ2022-03)。

摘　　要：为研究牦牛FGF1基因的生物学结构和功能,及其在不同组织中的表达规律,试验以桑桑牦牛心脏组织cDNA为模板,采用RT-PCR技术克隆了FGF1基因的CDS区序列,并对其进行生物信息学分析,利用实时荧光定量PCR(qRT-PCR)检测了FGF1基因在牦牛心脏、肝脏、脾脏、肺脏、肾脏、肌肉及脂肪组织中的表达水平。结果表明,桑桑牦牛FGF1基因的CDS区序列长468 bp,共编码155个氨基酸;相似性比对发现,桑桑牦牛与牛和瘤牛的同源性最高(99.8%),与鸡的同源性最低(73.7%);生物信息学分析表明,桑桑牦牛FGF1蛋白的理论等电点为6.51,为酸性亲水性蛋白,无信号肽和跨膜结构,主要定位在细胞核中;该蛋白存在7个糖基化位点和29处潜在的磷酸化位点;蛋白的二级结构主要以无规则卷曲为主,与FGF22、TGFB1和FGF17等蛋白存在主要相互作用。实时荧光定量结果显示,FGF1基因在桑桑牦牛各组织中均有不同程度的表达,其中在肺脏组织中的表达量极显著高于其他组织(P<0.01)。In order to study of the biological structure and function of the FGF1 gene in yak and its expression patterns in different tissues,the CDS region sequence of the FGF1 gene was cloned using RT-PCR technique with cDNA from the heart tissue of Sangsang yak as a template.Bioinformatics analysis was performed on the cloned sequence.The expression levels of the FGF1 gene in the heart,liver,spleen,lung,renal,muscle,and fat tissues of the yak were detected using real-time fluorescence quantitative PCR(qRT-PCR).The results showed that the CDS region sequence of the FGF1 gene in Sangsang yak was 468 bp,encoding a total of 155 amino acids.Similarity alignment revealed that Sang‐sang yak had the highest homology with Bos taurus and Bos indicus(99.8%),and the lowest homology with Gallus gallus(73.7%).Bioinformatics analysis indicated that the theoretical isoelectric point of the FGF1 protein in Sangsang yak was 6.51,indicating that it is an acidic and hydrophilic protein.It had no signal peptide and transmembrane structure,and was predominantly located in the cell nucleus.The protein possessed 7 glycosylation sites and 29 potential phosphorylation sites.The secondary structure of the protein was mainly composed of random coil structures and exhibited significant interactions with proteins such as FGF22,TGFB1 and FGF17.The results of real-time fluorescence quantitative analysis showed that the FGF1 gene was expressed in various tissues of Sangsang yak to different degrees,with the highest expression level in the lung tissue,significantly higher than in other tissues(P<0.01).

关 键 词：桑桑牦牛 FGF1基因 克隆 组织表达 

分 类 号：S823.85[农业科学—畜牧学]