基于网络药理学分析小承气汤治疗马肠梗阻的作用机制  

Analysis on the Mechanism of Xiaochengqi Decoction in the Treatment of Horse Intestinal Obstruction Based on Network Pharmacology

作　　者：尚立宏[1] 李宏胜[2] 韩庆彦[1] 白东东 SHANG Lihong;LI Hongsheng;Han Qingyan;BAI Dongdong(Gansu Provincial Animal Disease Prevention and Control Center,Lanzhou 730046,China;Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China;Beijing Centre Biology Co.,LTD.,Beijing 102600,China)

机构地区：[1]甘肃省动物疫病预防控制中心,兰州730046 [2]中国农业科学院兰州畜牧与兽药研究所,兰州730050 [3]北京生泰尔科技股份有限公司,北京102600

出　　处：《中国草食动物科学》2024年第3期23-32,共10页China Herbivore Science

基　　金：陇原青年创新创业人才项目(2023LQGR52);甘肃省农业农村厅科技项目(GNKJ-2020-29)。

摘　　要：肠梗阻是马最常见的疾病之一,小承气汤是治疗肠梗阻的经典方剂。研究旨在通过网络药理学分析阐明小承气汤治疗马肠梗阻的作用机制。首先在中药系统药理学数据库中检索了小承气汤的全部化学成分和靶标,然后利用STRING、DAVID、NCBI数据库和Cytoscape软件构建化合物-靶点网络、蛋白质-蛋白质相互作用(PPI)网络、靶点-通路网络,筛选小承气汤的活性化合物和靶标,建立治疗马肠梗阻的小承气汤网络图,研究分析小承气汤治疗马肠梗阻的GO、KEGG通路。结果表明,通过OB、DL和Caco-2筛选出16个有效化合物和32个对应靶标;小承气汤作用于马肠梗阻的PPI网络图包含45个靶点,主要关键靶点为TP53、ESR1、NCOA1、NCOA2、IGF1、MAPK14等,其中与马肠梗阻直接相关的靶点主要有TP53、ESR1、TGFB1、MMP9、HSP90AB1;共有24个基因本体(GO)条目和7条KEGG通路。说明小承气汤可能通过泽兰黄醇、决明内酯、胡萝卜苷_qt、β-谷甾醇、桉树醇、异橙黄酮等调控TP53、ESR1、NCOA1、NCOA2、IGF1、MAPK14等蛋白的表达。并通过肿瘤通路,MAPK信号通路,肿瘤蛋白聚糖,肿瘤坏死因子信号通路等治疗马肠梗阻。其作用机制有待进一步的生物学验证。Intestinal obstruction is one of the most common diseases in horses,and Xiaochengqi decoction is a classic formula for the treatment of intestinal obstruction.The aim of this study was to elucidate the mechanism of action of Xiaochengqi decoction for the treatment of equine intestinal obstruction by network pharmacological analysis.Firstly,all the chemical components and targets of Xiaochengqi decoction were searched in the systematic pharmacology database of traditional Chinese medicine,and then the compound-target network,protein-protein interaction(PPI)network,and target-pathway network were constructed by using STRING,DAVID,NCBI database,and Cytoscape software,to screen the active compounds and targets of Xiaochengqi decoction.The network diagram of Xiaochengqi decoction was constructed,and the GO and KEGG pathways of Xiaochengqi decoction in the treatment of equine intestinal obstruction were studied and analyzed.The results were as follows:16 effective compounds and 32 corresponding targets were screened by OB,DL and Caco-2;the PPI network map of Xiaochengqi decoction acting on equine intestinal obstruction contained 45 targets,and the main key targets were TP53,ESR1,NCOA1,NCOA2,IGF1,MAPK14,etc,among which the targets directly related to equine intestinal obstruction were mainly TP53,ESR1,TGFB1,MMP9,HSP90AB1;there were 24 gene ontology(GO)entries and 7 KEGG pathways.The present study suggests that Xiaochengqi decoction may regulate the expression of proteins such as TP53,ESR1,NCOA1,NCOA2,IGF1,and MAPK14 through zeylanaxanthin,cassia lactone,carotenoid qt,β-sitosterol,eudesmol,and isohespergone.It also treats equine intestinal obstruction through the tumour pathway,MAPK signalling pathway,tumour proteoglycan,and tumour necrosis factor signalling pathway.The mechanism of action needs further biological validation.

关 键 词：网络药理学 小承气汤 马肠梗阻 

分 类 号：S853.7[农业科学—临床兽医学]