西门塔尔牛输精后21天彩色多普勒预判妊娠方法的建立  

Establishment of Method to Predict Pregnancy by Color Doppler at 21 Days After Insemination in Simmental Cattle

作　　者：艾力亚木·土尔衮 常站站 古力孜巴·艾力 李伟建 梁亦白 王莉 刘娅兰 王旭光[1] Ailiyamu·Tuergun;CHANG Zhanzhan;Guliziba·Aili;LI Weijian;LIANG Yibai;WANG Li;LIU Yalan;WANG Xuguang(College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052

出　　处：《中国草食动物科学》2024年第3期45-51,共7页China Herbivore Science

基　　金：2024年自治区现代畜禽种业提升专项(2024XM-22)。

摘　　要：为建立应用彩色多普勒开展黄体血流评估、预判西门塔尔牛在配后21 d妊娠情况的方法,选择98头西门塔尔牛进行定时输精,配后21 d使用B超、彩色多普勒进行卵巢检查,采集黄体的最大面积、黄体血流面积;同时采集血样分析激素水平,在配后35 d进行B超孕检,对比分析妊娠情况。结果表明,在配后21 d通过主观预判检出8头空怀牛,以血流面积为预判标准预判检出26头空怀牛,以血流黄体比预判标准预判检出23头空怀牛,配后35 d B超检查出36头空怀牛;妊娠牛孕酮(P_(4))、雌二醇比孕酮(E_(2)/P_(4))水平均极显著高于空怀牛(P<0.01);使用血流黄体比预判标准对空怀牛的灵敏性为100%,可以用该方法尽早发现空怀牛,相较于配后30~35 d B超检查妊娠,配后21 d彩色多普勒检查可以让空怀牛提前9~15 d再次进行定时输精。The purpose of this paper was to establish a method for using color Doppler to evaluate luteal blood flow and predict pregnancy status of Simental cow at 21 days after insemination.98 Simental cows were selected for timed artificial insemination(AI).The ovaries were examined by B type ultrasound and color Doppler at 21 days after insemination,and the maximum area of the corpus luteum and the area of corpus luteumblood flow were collected;at the same time,blood samples were collected to analyze the hormone level,and ultrasound pregnancy determination was performed at 35 days after insemination.The results showed that 8 unpregnant cows were detected by subjective prejudgement at 21 days after AI,26 unpregnant cows were checked out under the blood flow area as a criterion,23 unpregnant cows were detected with blood flow luteal ratio prejudgement,and 36 unpregnant cows were detected by ultrasound examination at 35 days after AI;the levels of progesterone,and ratio of estradiol to progesterone in pregnant cows were significant higher than those in unpregnant cows(P<0.01);the prognostic criterion of the blood flow area luteal area ratio was 100%sensitive for unpregnant cows,nulliparous cows can be detected as early as possible by this method,and compared with ultrasound examination of pregnancy 30-35 days after AI,color Doppler examination 21 days after AI allows unpregnant cows to undergo timed artificial insemination again 9-15 days earlier.

关 键 词：西门塔尔牛 早孕检查 彩色多普勒 曲线下面积 空怀预判 

分 类 号：S823.3[农业科学—畜牧学]