三代测序和单细胞转录组测序技术在家畜雄性生殖领域的研究进展  

Research Progress of Third-generation Sequencing and Single-cell Transcriptome Technology in the Field of Livestock Male Reproduction

作　　者：王兴东 郭宪[1] WANG Xingdong;GUO Xian(Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Key Laboratory of Yak Breeding of Gansu Province,Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau,Ministry of Agriculture and Rural Affairs,Lanzhou 730050,China)

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,甘肃省牦牛繁育重点实验室,农业农村部青藏高原畜禽遗传育种重点实验室,兰州730050

出　　处：《中国草食动物科学》2024年第3期59-65,共7页China Herbivore Science

基　　金：财政部和农业农村部-现代农业(肉牛牦牛)产业技术体系建设专项(CARS-37);中国农业科学院科技创新工程(25-LZIHPS-01);夏河县东西部科技协作项目(202301)。

摘　　要：雄性家畜优良的繁殖性能可以提高养殖效益,促进家畜种群的改良和生产性能的提升,而睾丸正常发育是雄性哺乳动物精子发生及有效繁殖力的先决条件。近年来,高通量测序技术已广泛应用于哺乳动物睾丸发育及精子发生的各项研究中。其中三代测序和单细胞转录组测序技术已经成为深入研究家畜雄性生殖及精子发生机制的有效工具。文章综述了雄性睾丸发育,三代测序和单细胞转录组测序技术及其在睾丸发育中的应用和研究进展,揭示出与雄性生殖发育相关的关键基因和途径,为提高家畜的繁殖效率及研究生殖疾病的病因提供参考依据。The excellent reproductive performance of male livestock can improve the breeding efficiency,promote the improvement of livestock population and increase the production performance,and the normal development of testis is a prerequisite for the spermatogenesis and effective reproduction of male mammals.In recent years,high-throughput sequencing technology has been widely used in the studies of testicular development and spermatogenesis in mammals.The third-generation sequencing and single-cell transcriptome sequencing techniques have become effective tools for in-depth research on the mechanisms of male reproduction and spermatogenesis in livestock.This paper reviewed male testicular development,as well as third-generation sequencing and single-cell transcriptome sequencing technology and their application and research progress in testicular development,revealing the key genes and pathways related to male reproductive development,and providing the reference for improving reproductive efficiency and studying the causes of reproductive diseases of domestic animals.

关 键 词：三代测序 单细胞转录组 雄性生殖 

分 类 号：S337[农业科学—作物遗传育种]