Overexpression of the transcription factor MdWRKY115 improves drought and osmotic stress tolerance by directly binding to the MdRD22 promoter in apple  

作　　者：Qinglong Dong Yi Tian Xuemei Zhang Dingyue Duan He Zhang Kaiyu Yang Peng Jia Haoan Luan Suping Guo Guohui Qi Ke Mao Fengwang Ma 

机构地区：[1]College of Forestry,Hebei Agricultural University,Baoding,Hebei 071001,China [2]National Engineering Research Center for Agriculture in Northern Mountainous Areas,Agricultural Technology Innovation Center in Mountainous Areas of Hebei Province,Hebei Agricultural University,Baoding,Hebei 071001,China [3]State Key Laboratory of Crop Stress Biology for Arid Areas,Shaanxi Key Laboratory of Apple,College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China [4]College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071001,China

出　　处：《Horticultural Plant Journal》2024年第3期629-640,共12页园艺学报（英文版）

基　　金：supported by grants from the Natural Science Foundation of Hebei Province(Grant No.C2022204086);the Hebei Apple Innovation Team of Modern Agricultural Industry Technology System(Grant No.HBCT2021100211);the National Natural Science Foundation of China(Grant No.32072524).

摘　　要：Abiotic stress reduces plant yield and quality.WRKY transcription factors play key roles in abiotic stress responses in plants,but the molecular mechanisms by which WRKY transcription factors mediate responses to drought and osmotic stresses in apple(Malus×domestica Borkh.)remain unclear.Here,we functionally characterized the apple GroupⅢWRKY gene MdWRKY115.qRT-PCR analysis showed that MdWRKY115 expression was up-regulated by drought and osmotic stresses.GUS activity analysis revealed that the promoter activity of MdWRKY115 was enhanced under osmotic stress.Subcellular localization and transactivation assays indicated that MdWRKY115 was localized to the nucleus and had a transcriptional activity domain at the N-terminal region.Transgenic analysis revealed that the overexpression of MdWRKY115 in Arabidopsis plants and in apple callus markedly enhanced their tolerance to drought and osmotic stresses.DNA affinity purification sequencing showed that MdWRKY115 binds to the promoter of the stress-related gene MdRD22.This binding was further verified by an electrophoretic mobility shift assay.Collectively,these findings suggest that MdWRKY115 is an important regulator of osmotic and drought stress tolerance in apple.

关 键 词：APPLE MdWRKY115 Osmotic stress Drought stress Function analysis 

分 类 号：S661.1[农业科学—果树学]