Comparative transcriptome analysis reveals key genes and pathways in response to Alternaria alternata apple pathotype infection  

作　　者：Kai Liu Zhaolin Liang An Yang Jiadi Yan Peihua Cong Xiaolei Han Caixia Zhang 

机构地区：[1]Key Laboratory of Horticultural Crop Germplasm Utilization of the Ministry of Agriculture and Rural Affairs(MOA),Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng,Liaoning 125100,China

出　　处：《Horticultural Plant Journal》2024年第3期641-656,共16页园艺学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Grant No.32202463);China Agriculture Research System(Grant No.CARS-27);the Agricultural Science and Technology Innovation Program(Grant No.CAAS-ASTIP-2021-RIP-02)。

摘　　要：Apple leaf spot,caused by the Alternaria alternata apple pathotype(AAAP),is an important fungal disease of apple.To understand the molecular basis of resistance and pathogenesis in apple leaf spot,the transcriptomes of two apple cultivars‘Hanfu'(HF)(resistant)and‘Golden Delicious'(GD)(susceptible)were analyzed at 0,6,18,24 and 48 h after AAAP inoculation by RNA-Seq.At each time point,a large number of significantly differentially expressed genes(DEGs)were screened between AAAP-inoculated and uninoculated apple leaves.Analysis of the common DEGs at four time points revealed significant differences in the resistance of‘HF'and‘GD'apple to AAAP infection.RLP,RNL,and JA signal-related genes were upregulated in both cultivars to restrict AAAP development.However,genes encoding CNLs,TNLs,WRKYs,and AP2s were only activated in‘HF'as part of the resistance response,of which,some play major roles in the regulation of ET and SA signal transduction.Further analysis showed that many DEGs with opposite expression trends in the two hosts may play important regulatory roles in response to AAAP infection.Transient expression of one such gene MdERF110 in‘GD'apple leaves improved AAAP resistance.Collectively,this study highlights the reasons for differential resistance to AAAP infection between‘HF'and‘GD'apples which can theoretically assist the molecular breeding of disease-resistant apple crops.

关 键 词：APPLE TRANSCRIPTOME Alternaria alternata apple pathotype Infection Defense response 

分 类 号：S436.611.1[农业科学—农业昆虫与害虫防治]