Synthesis of betanin by expression of the core betalain biosynthetic pathway in carrot  

作　　者：Bo Wang Yahui Wang Yuanjie Deng Quanhong Yao Aisheng Xiong 

机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China [2]Shanghai Key Laboratory of Agricultural Genetics and Breeding,Biotechnology Research Institute of Shanghai Academy of Agricultural Science,Shanghai 201106,China

出　　处：《Horticultural Plant Journal》2024年第3期732-742,共11页园艺学报（英文版）

基　　金：supported by National Natural Science Foundation of China(Grant No.32072563);Key Research and Development Projects of Ningxia Hui Autonomous Region(Grant No.2022BBF02008);Key Project Fund of the Shanghai Municipal Committee of Agriculture(Grant Nos.2021-02-08-00-12-F00795,Chuangzi 20181e5);Shanghai Academic Technology Research Leader(Grant No.19XD1432300);Priority Academic Program Development of Jiangsu Higher Education Institutions Project(PAPD)。

摘　　要：Betalain has received increased attention because of its high nutritional value and crucial physiological functions.Based on the elucidation of its core biosynthetic pathway,betalain can be produced in additional plants by metabolic engineering.Synthesis of betalain in carrot(Daucus carota L.)can improve its nutritional quality and economic value by extracting betalain from the fleshy root,non-edible part,and processing residue of carrot.In this study,two different constructs,namely,pYB:mCD(AomelOS,BvCYP76AD1S,and BvDODA1S)and p YB:CDD(BvCYP76AD1S,BvDODA1S,and MjcDOPA5GTS),were introduced into carrot for betanin synthesis by Agrobacterium-mediated transformation.Betanin can be synthetized in both transgenic calli,and p YB:m CD-transgenic callus can be used to produce betacyanin by suspension culture.However,pYB:mCD-transgenic seedlings can synthetize betanin only by tyrosine feeding.The p YB:CDD-transgenic lines can synthetize betanin in whole plants.The betanin content in fleshy root of pYB:CDD-transgenic carrot was(63.4±9)μg·g^(-1)fresh weight according to quantitative analysis.These betanin-producing carrot plant materials can be used to synthesize betanin for industrial application or consumption as dietary sources.

关 键 词：Daucus carota L. BETANIN Biosynthesis Metabolic engineering BIOFORTIFICATION 

分 类 号：S631.2[农业科学—蔬菜学]