DIA-based proteome profiling with PRM verification reveals the involvement of ER-associated protein processing in pollen abortion in Ogura CMS cabbage  

作　　者：Peiwen Wang Lin Zhu Ziheng Li Mozhen Cheng Xiuling Chen Aoxue Wang Chao Wang Xiaoxuan Zhang 

机构地区：[1]Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs,Northeast Agricultural University,Harbin,Heilongjiang 150030,China [2]College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin,Heilongjiang 150030,China

出　　处：《Horticultural Plant Journal》2024年第3期755-770,共16页园艺学报（英文版）

基　　金：supported by Heilongjiang Provincial Natural Science Foundation of China(Grant No.YQ2022C012);China Postdoctoral Science Foundation(Grant No.2022MD713728);Heilongjiang Provincial Postdoctoral Fund(Grant No.LBHZ21046);the Open Project of Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs,and National Key Research and Development Program of China(Grant No.2023YFD1201501).

摘　　要：Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a data-independent acquisition-based proteomic approach coupled with a parallel reaction monitoring-based targeted proteomic assay was used to analyze the proteome dynamics of Ogura CMS cabbage line RM and its maintainer line RF during floral bud development to obtain insights into the mechanism underlying Ogura CMS in cruciferous crops.A total of 9162 proteins corresponding to 61464 peptides were identified in RM and RF floral buds.The proteomic fluctuation of RM was weaker than that of RF.Differences in protein expression between RM and RF gradually enlarged with floral bud development.Fifteen continually up-regulated and eight continually down-regulated proteins were found in RM relative to RF throughout floral bud development.Differentially expressed proteins between RM and RF during floral bud development were implicated in the endoplasmic reticulum(ER)-associated protein processing pathway,in which most of them exhibited down-regulated expression in RM.These data suggest that ER-associated protein processing may be involved in pollen abortion in Ogura CMS cabbage by inhibiting the expression of critical factors.Our findings not only deepen the understanding of the molecular mechanisms of Ogura CMS in cruciferous crops but also provide better guidance for applying Ogura CMS in the hybrid breeding of cruciferous crops.

关 键 词：Ogura cytoplasmic male sterility(Ogura CMS) CABBAGE Data-independent acquisition(DIA) Parallel reaction monitoring(PRM) Pollen development 

分 类 号：S635[农业科学—蔬菜学]