BnaWRKY75 positively regulates the resistance against Sclerotinia sclerotiorum in ornamental Brassica napus  

作　　者：Kexin Yu Yijie Zhang Xiaoyuan Fei Luyue Ma Rehman Sarwar Xiaoli Tan Zheng Wang 

机构地区：[1]School of Life Sciences,Jiangsu University,Zhenjiang,Jiangsu 212013,China [2]School of Food and Biological Engineering,Jiangsu University Zhenjiang,Jiangsu 212013,China [3]Key Laboratory for Theory and Technology of Intelligent Agricultural Machinery and Equipment,Jiangsu University,Zhenjiang,Jiangsu 212013,China

出　　处：《Horticultural Plant Journal》2024年第3期784-796,共13页园艺学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.31771836);the National Key Research and Development Program of China(Grant No.2018YFD0201003).

摘　　要：With the development of tourism at home and abroad,Rapeseed(Brassica napus)has become an important ornamental plant.However,its ornamental value at the inflorescence stage is greatly reduced by Sclerotinia sclerotiorum.Identification of important genes in the defense responses is critical for molecular breeding,which is an important strategy for controlling the disease.In this study,we isolated a B.napus WRKY transcription factor gene,BnaWRKY75.BnaWRKY75 was found to encode a nucleus-localized protein and exhibited relatively high expression in the stems.Arabidopsis thaliana transgenic plants expressing BnaWRKY75 showed enhanced resistance to S.sclerotiorum,and both ProBnaWRKY75:GUS and gene expression analyses showed that BnaWRKY75 was highly responsive to S.sclerotiorum infection,indicating the involvement of BnaWRKY75 in response to this infection.Furthermore,overexpression(OE)of BnaWRKY75 in B.napus significantly enhanced the resistance to S.sclerotiorum,whereas the resistance was reduced in RNAi transgenic B.napus plants.Moreover,the BnaWRKY75-OE B.napus plants exhibited constitutive activation of salicylic acid-,jasmonic acid-,and ethylene-mediated defense responses and the inhibition of both H_(2)O_(2)and O_(2)·^(-)accumulation in response to this pathogen.By contrast,BnaWRKY75-RNAi plants showed a reverse pattern,suggesting that BnaWRKY75 is involved in hormonal signaling pathways and in the control of reactive oxygen species accumulation.In conclusion,these data indicate that BnaWRKY75,a regulator of multiple defense responses,positively regulates resistance against S.sclerotiorum,which may guide the improvement of resistance in rapeseed.

关 键 词：Brassica napus BnaWRKY75 Defense response Sclerotinia sclerotiorum 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]