RAD-seq data reveals robust phylogeny and morphological evolutionary history of Rhododendron  

作　　者：Yuanting Shen Gang Yao Yunfei Li Xiaoling Tian Shiming Li Nian Wang Chengjun Zhang Fei Wang Yongpeng Ma 

机构地区：[1]Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,Yunnan 650201,China [2]Key Laboratory for Plant Diversity and Biogeography of East Asia,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,Yunnan 650201,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]Beijing Genomics of Institute,Shenzhen,Guangdong 518120,China [5]Guiyang Institute of Humanities and Technology,Guiyang,Guizhou 550025,China [6]State Forestry and Grassland Administration Key Laboratory of Silviculture in Downstream Areas of the Yellow River,College of Forestry,Shandong Agricultural University,Tai'an,Shandong 271018,China [7]The Southwest China of Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,Yunnan 650201,China [8]West China Subalpine Botanical Garden,Institute of Botany,Chinese Academy of Sciences,Chengdu,Sichuan 611834,China

出　　处：《Horticultural Plant Journal》2024年第3期866-878,共13页园艺学报（英文版）

基　　金：supported by Ten Thousand Talent Program of Yunnan Province(Grant No.YNWR-QNBJ-2018-174);the Key Basic Research Program of Yunnan Province,China(Grant No.202101BC070003);National Natural Science Foundation of China(Grant No.31901237);Conservation Program for Plant Species with Extremely Small Populations in Yunnan Province(Grant No.2022SJ07X-03);Key Technologies Research for the Germplasmof Important Woody Flowers in Yunnan Province(Grant No.202302AE090018);Natural Science Foundation of Guizhou Province(Grant No.Qiankehejichu-ZK2021yiban 089&Qiankehejichu-ZK2023yiban 035)。

摘　　要：Rhododendron is famous for its high ornamental value.However,the genus is taxonomically difficult and the relationships within Rhododendron remain unresolved.In addition,the origin of key morphological characters with high horticulture value need to be explored.Both problems largely hinder utilization of germplasm resources.Most studies attempted to disentangle the phylogeny of Rhododendron,but only used a few genomic markers and lacked large-scale sampling,resulting in low clade support and contradictory phylogenetic signals.Here,we used restriction-site associated DNA sequencing(RAD-seq)data and morphological traits for 144 species of Rhododendron,representing all subgenera and most sections and subsections of this species-rich genus,to decipher its intricate evolutionary history and reconstruct ancestral state.Our results revealed high resolutions at subgenera and section levels of Rhododendron based on RAD-seq data.Both optimal phylogenetic tree and split tree recovered five lineages among Rhododendron.Subg.Therorhodion(cladeⅠ)formed the basal lineage.Subg.Tsutsusi and Azaleastrum formed cladeⅡand had sister relationships.CladeⅢincluded all scaly rhododendron species.Subg.Pentanthera(cladeⅣ)formed a sister group to Subg.Hymenanthes(cladeⅤ).The results of ancestral state reconstruction showed that Rhododendron ancestor was a deciduous woody plant with terminal inflorescence,ten stamens,leaf blade without scales and broadly funnelform corolla with pink or purple color.This study shows significant distinguishability to resolve the evolutionary history of Rhododendron based on high clade support of phylogenetic tree constructed by RAD-seq data.It also provides an example to resolve discordant signals in phylogenetic trees and demonstrates the application feasibility of RAD-seq with large amounts of missing data in deciphering intricate evolutionary relationships.Additionally,the reconstructed ancestral state of six important characters provides insights into the innovation of key characters in Rhododend

关 键 词：RHODODENDRON RAD-seq Missing data Quartet sampling(QS) Ancestral state reconstruction 

分 类 号：S685.21[农业科学—观赏园艺]