Lipase and photodecarboxylase coexpression: A potential strategy for alkane-based biodiesel production from natural triglycerides  

作　　者：Yong-Yi Zeng Xin-Yi Xu Jin-Xuan Xie Wen-Li Chen Lan Liu Xin-Jian Yin Bi-Shuang Chen 

机构地区：[1]School of Marine Sciences,Sun Yat-Sen University,Zhuhai 519080,China [2]Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai 519080,China

出　　处：《Chinese Journal of Chemical Engineering》2024年第3期238-246,共9页中国化学工程学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(42376097);Guangdong Basic and Applied Basic Research Foundation(2023A1515030226,2021A1515010829).

摘　　要：Alkane-based biodiesel is considered the next generation of biodiesel owing to its potential environmental benefits and the fact that it exhibits much higher specific caloric values than traditional biodiesel.However,the formidable obstacle impeding the commercialization of this cutting-edge fuel alternative lies in the cost associated with its production.In this study,an engineered strain Escherichia coli(E.coli)showcasing harmonized coexpression of a lipase(from Thermomyces lanuginosus lipase,TLL)and a fatty acid photodecarboxylase(from Chlorella variabilis,CvFAP)was first constructed to transform triglycerides into alkanes.The potential of E.coli BL21(DE3)/pRSFDuet-1-TLL-CvFAP for alkane synthesis was evaluated with tripalmitin as a model substrate under various process conditions.Following a comprehensive examination of the reaction parameters,the scope of the biotransformation was expanded to‘real’substrates(vegetable oils).The results showed that bioderived oils can be transformed into alkanes with high yields(0.80-10.20 mmol·L^(-1))under mild conditions(35℃,pH 8.0,and 36 h)and blue light illumination.The selected processes were performed on an increased lab scale(up to 100 ml)with up to 24.77 mmol·L^(-1) tripalmitin,leading to a yield of 18.89 mmol·L^(-1) pentadecane.With the employment of a method for efficiently producing alkanes under mild conditions and a simple procedure to isolate alkanes from the reaction system,the utilization of sustainable biomass as a fundamental feedstock emerges as the primary solution to lower the cost of alkane-based biodiesel.Thus,this study proposes a readily implementable and highly effective approach for alkane-based biodiesel production.

关 键 词：Alkane-based biodiesel Renewable biomass Co-overexpression LIPASE Photodecarboxylase 

分 类 号：TE667[石油与天然气工程—油气加工工程]