尖孢镰刀菌亚麻专化型FolGLS2基因功能分析  

Research on the function of FolGLS2 gene in Fusarium oxysporum f.sp.lini

作　　者：高伟娜 侯占铭[1] GAO Wei-na;HOU Zhan-ming(College of Life Science and Technology,Inner Mongolia Normal University,Key Laboratory of Biodiversity Conserva-tion and Sustainable Utilization,Advanced Universities of Inner Mongolia,Hohhot 010022,China)

机构地区：[1]内蒙古师范大学生命科学与技术学院,内蒙古自治区高等学校生物多样性保护与可持续利用重点实验室,内蒙古呼和浩特010022

出　　处：《中国油料作物学报》2024年第2期294-302,共9页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31160280);内蒙古自治区自然科学基金(2015MS0311)。

摘　　要：亚麻是我国主要的油料作物,且长期受亚麻枯萎病的侵害。β-1,3-葡聚糖合酶的催化亚基FKS/GLS为细胞壁合成所必需,本研究为鉴定尖孢镰刀菌亚麻专化型FolGLS2的功能,进行了FolGLS2基因的克隆、敲除以及敲除突变体表型和致病力的测定等试验。结果显示,FolGLS2基因全长5947 bp,cDNA开放阅读框为5847 bp,编码1948个氨基酸。表型观察发现,敲除突变体菌落小而紧实,生长速率明显降低;菌丝缩短、增粗且无法产生分生孢子;菌丝细胞壁对于常规原生质释放酶液具有抗性,无法释放原生质体;感染试验显示,突变体致病力明显减弱。上述结果表明,FolGLS2基因编码β-1,3-葡聚糖合成酶催化亚基,对菌丝营养生长、菌落形态、分生孢子发生、细胞壁组成及致病性具有调控作用。Flax is the main oil crop in China,and has been affected by flax wilt for a long time.The catalytic subunit ofβ-1,3-glucan synthase(FKS/GLS)is necessary for cell wall synthesis,the biological function of which was analyzed in the study through cloning the gene,constructing FolGLS2 gene deletion mutant,observing phenotype and assaying the virulence of the gene disruption mutants.A full-length of 5947bp DNA sequence was obtained,which contained an open reading frame 5847bp,encoding a total of 1948 amino acids.Observation of the phenotype of the FolGLS2 gene deletion mutant showed that the colony of the mutant was small and compact,its growth rate decreased obviously,the mycelium was short,thick and no longer produced conidia.The cell wall of the mutant mycelium changed somehow,which was strongly resistant to the protoplasting buffer commonly used for wild type and was unable to release protoplasts.Infection assay to the flax seedling demonstrated that pathogenicity of deletion mutant was significantly decreased.Conclusively,FolGLS2 gene,which encodes catalytic subunit ofβ-1,3-glucan synthase,plays a regulatory role in conidiogenesis,mycelial vegetative growth,cell wall properties and pathogenicity of Fusarium oxysporum f.sp.lini.

关 键 词：尖孢镰刀菌亚麻专化型 Split-Marker技术 FolGLS2 基因敲除 

分 类 号：S435.63[农业科学—农业昆虫与害虫防治]