印度梨形孢-花生共生体系建立及对花生的促生作用  

Establishment of Piriformospora indica-peanut symbiosis system and its promoting effect on peanut growth

作　　者：高洁 田培聪 党悦嘉 李昕芮 王趁 刘闯 黄玉茜[2] GAO Jie;TIAN Pei-cong;DANG Yue-jia;LI Xin-rui;WANG Chen;LIU Chuang;HUANG Yu-qian(College of Land and Environment,Shenyang Agricultural University,Shenyang 110866,China;College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,China;College of Plant Sciences,Jilin University,Changchun 130062,China)

机构地区：[1]沈阳农业大学土地与环境学院,辽宁沈阳110866 [2]沈阳农业大学植物保护学院,辽宁沈阳110866 [3]吉林大学植物科学学院,吉林长春130062

出　　处：《中国油料作物学报》2024年第2期385-393,共9页Chinese Journal of Oil Crop Sciences

基　　金：国家重点研发计划(2018YFD0201007-2);辽宁省普通高校基本科研面上项目(LJKMZ20221044);辽宁省北镇市花生科技特派团项目(2022JH5/10400053)。

摘　　要：印度梨形孢(Piriformospora indica)是一种内生真菌。为提高花生产量,探究印度梨形孢对花生的促生作用,以花生(粤油7号)为材料,采用室外盆栽实验,建立印度梨形孢与花生共生体系,调查对花生植株的农艺性状、产量、光合特性、丙二醛含量、根系活力和抗氧化酶活性等生理指标的影响。结果发现:采用菌丝浇灌的方式接种印度梨形孢,成功建立了与花生的共生体系,侵染率为63%。苗期时,叶片叶绿素SPAD值和净光合速率显著增加24.17%和19.66%,蒸腾速率比未接种印度梨形孢的花生植株22%;在苗期与成熟期,接种印度梨形孢的花生主根长、地下部鲜重、地下部干重、根系活力和叶片的过氧化氢酶(CAT)、过氧化物酶(POD)活性显著提高,丙二醛含量分别显著降低24.27%和22.12%;接种印度梨形孢提高了花生产量。To improve peanut yield,growth-promoting effect of endophytic fungus Piriformospora indica on peanut plants was investigated in pots.Peanut cv.Yueyou 7 was used as experimental material.Symbiosis system between P.indica and peanut was established.Agronomic traits,yield,photosynthetic characteristics,malondialdehyde content,root activity and antioxidant enzyme activity of peanut were studied.Results showed that the symbiosis system of P.indica and peanut was established successfully by inoculating P.indica with hyphae.The infection rate was 63%.At seedling stage,net photosynthetic rate and chlorophyll SPAD value of peanut leaves were significantly increased by 24.17%and 19.66%,and transpiration rate was 22%lower than that of uninoculated peanut plants.At seedling and mature stages,compared with uninoculated P.indica,root length,underground fresh weight,underground dry weight,root activity and leaf catalase peroxidase activity were significantly increased after inoculation.Content of malondialdehyde was significantly reduced by 24.27%and 22.12%at seedling and mature stages.Peanut yield was increased by inoculation with P.indica.

关 键 词：花生 印度梨形孢 农艺性状 抗氧化酶 产量 

分 类 号：S565.2[农业科学—作物学]