水培条件下花生品种铁素吸收/活化能力鉴定方法  

Identification method of ferritin absorption/activation ability of peanut(Arachis hypogaea L.)varieties under hydroponic condition

作　　者：张静 骆璐 刘风珍[1] 贾红霞 刘娟[2] 谭震 张昆 万勇善[1] ZHANG Jing;LUO Lu;LIU Feng-zhen;JIA Hong-xia;LIU Juan;TAN Zhen;ZHANG Kun;WAN Yong-shan(State Key Laboratory of Crop Biology/College of Agronomy,Shandong Agricultural University,Tai'an 271018,China;Institute of Cash Crops,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;China Inspection and Certification Group Shandong Co.,LTD.,Qingdao 266000,China)

机构地区：[1]山东农业大学作物生物学国家重点实验室/山东农业大学农学院,山东泰安271018 [2]河南省农业科学院经济作物研究所,河南郑州450002 [3]中国检验认证集团山东有限公司,山东青岛266000

出　　处：《中国油料作物学报》2024年第2期411-419,共9页Chinese Journal of Oil Crop Sciences

基　　金：国家重点研发计划(SQ2018YFD100007);山东省农业良种工程项目(2020LZGC001)。

摘　　要：为建立快速鉴定不同花生品种铁素吸收、活化能力的方法,以耐缺铁花生品种农大818、鲁花11、山花11号,铁敏感花生品种鲁花12、ICG6848、白沙1016为材料进行水培试验。以缺铁营养液培养花生幼苗,观察并记录在缺铁环境中幼苗新叶黄化时间;待幼苗新叶全部黄化后恢复供铁,分别以20μmol/L FeSO_(4)处理2 d/4 d,25μmol/L FeSO_(4)处理2 d/3 d,30μmol/L FeSO_(4)处理1 d/2 d/4 d,通过测定叶绿素值增加量(ΔSPAD)、干物重、铁吸收量等指标以鉴定花生铁素吸收能力;以25μmol/L Fe_(2)(SO_(4))_(3)处理2 d/4 d,30μmol/L Fe_(2)(SO_(4))_(3)处理2 d/3 d,35μmol/L Fe_(2)(SO_(4))_(3)处理1 d/2 d/4 d以鉴定花生铁素活化能力。结果表明,所有花生品种的幼苗在缺铁处理第10 d均出现了黄化现象,此时用25μmol/L FeSO_(4)恢复供铁处理2 d后,耐缺铁品种和铁敏感品种间ΔSPAD、单株铁吸收量差异显著;用30μmol/L Fe_(2)(SO_(4))_(3)处理2 d后,耐缺铁品种和铁敏感品种间ΔSPAD、单株铁活化量也有显著差异。因此可在缺铁黄化后补充25μmol/L FeSO_(4)或30μmol/L Fe_(2)(SO_(4))_(3),用处理2 d时花生幼苗ΔSPAD、单株铁吸收量或单株活化量作为评价花生铁素吸收/活化能力的指标。In order to establish a rapid method to identify the absorption and activation ability of iron in different peanuts,the iron deficiency cultivars Nongda 818,Luhua 11,Shanhua 11,and the iron sensitive cultivars Luhua 12,ICG6848,and Baisha 1016 were employd in iron deficiency nutrient solution instead of soil.The yellowing time of new leaves in iron deficiency environment was recorded.After the new leaves of all peanut seedlings turned yellow,the iron supply was resumed.The seedlings were treated with 20µmol/L FeSO_(4)for 2 d and 4 d,25µmol/L FeSO_(4)for 2 d and 3 d,and 30µmol/L FeSO_(4)for 1 d,2 d and 4 d,the increase of chlorophyll value(ΔSPAD),dry weight and iron uptake were measured to identify the iron uptake capacity.The iron activation ability of peanut was evaluated at 25µmol/L Fe_(2)(SO_(4))_(3) for 2 d and 4 d,30µmol/L Fe_(2)(SO_(4))_(3) for 2 and 3 d,and 35µmol/L Fe_(2)(SO_(4))_(3) for 1 day,2 d and 4 d.Results showed that the seedlings of all peanuts showed yellowing on the 10th day of iron deficiency treatment.Then,25µmol/L FeSO_(4)was used to iron supply for 2 days,which led to increase of SPAD value,while iron uptake per plant were significantly different between iron deficiency tolerant and iron sensitive cultivars.When treated with 30µmol/L Fe_(2)(SO_(4))_(3) for 2 days,the increase of SPAD value and iron activation per plant were also significantly different between the deficient tolerant and sensitive cultivars.Therefore,considerting peanut iron absorption/activation ability,the increase of seedling SPAD,iron uptake per plant and/or activation per plant could be used as indicators after iron supply(25µmol/L FeSO_(4)or 30µmol/L Fe_(2)(SO_(4))_(3) for 2 days)when seedlings have been etiolated.

关 键 词：花生 铁 水培 吸收 活化 

分 类 号：S565.2[农业科学—作物学]