鸢尾黄素对前脂肪细胞分化的抑制作用  

Inhibitory effects of tectorigenin on the differentiation of preadipocytes

作　　者：潘婷 高洁 张会会 蔡兴华 雷涛[1] 鲁郡 PAN Ting;GAO Jie;ZHANG Hui-hui;CAI Xing-hua;LEI Tao;LU Jun(Division of Endocrinology,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China;School of Medical and Life Sciences,Chengdu University of Traditional Chinese Medicine,Chengdu 610000,China;Shanghai Putuo Central School of Clinical Medicine,Anhui Medical University,Shanghai 200062,China)

机构地区：[1]上海中医药大学附属普陀医院内分泌科,上海200062 [2]成都中医药大学医学与生命科学学院,四川成都610000 [3]安徽医科大学上海普陀中心临床学院,上海200062

出　　处：《中成药》2024年第5期1492-1498,共7页Chinese Traditional Patent Medicine

基　　金：上海市医学重点专科(ZK2019B16);上海市卫生健康委员会科研课题(20204Y0154,202240309);上海市普陀区临床特色专科(2020tszk01);上海市普陀区卫生健康系统科技创新项目(ptkwws202003)。

摘　　要：目的探索鸢尾黄素对前脂肪细胞分化的影响及机制。方法CCK8法检测鸢尾黄素对3T3-L1细胞存活率的影响。细胞接触抑制2 d(分化第0天),加入诱导剂和不同浓度鸢尾黄素(0、10、20、40、60μmol/L)处理细胞,对照组不加诱导剂。分化第9天,油红O染色观察脂滴形成,生化试剂盒检测TG、NEFA水平,Western blot法检测PPARγ、C/EBPα、perilipin-1、ADFP、AMPKα、p-AMPKα蛋白表达,RT-qPCR法检测Adiponectin、FABP4、FAS、Acly mRNA表达。结果鸢尾黄素浓度≤60μmol/L时不影响细胞存活率(P>0.05)。与诱导分化组比较,鸢尾黄素给药后,细胞内脂滴形成减少,TG、NEFA水平降低(P<0.01);C/EBPα、PPARγ、perilipin-1、ADFP蛋白表达降低(P<0.01),AMPKα、p-AMPKα蛋白表达升高(P<0.01);Adiponectin、FABP4、FAS、Acly mRNA表达降低(P<0.01)。结论鸢尾黄素具有抑制前脂肪细胞分化为成熟脂肪细胞的作用,其机制可能与C/EBPα、PPARγ、AMPKα和p-AMPKα的调控有关。AIM To investigate the effects of tectorigenin on preadipocyte differentiation and the possible mechanism.METHODS CCK8 method was used to detect the effects of tectorigenin on 3T3-L1 cell viability.After 2 days contact inhibition(Day 0 of differentiation),the cells were exposed to inducer and tectorigenin of different concentrations(0,10,20,40,60μmol/L),in contrast to those of the control group with no inducer use.On the 9th day of differentiation,the cells had their lipid droplets observed by oil red O staining;their levels of TG and NEFA detected by biochemical kit;their protein expressions of PPARγ,C/EBPα,perilipin-1,ADFP,AMPKαand p-AMPKαdetected by Western blot;and their mRNA expressions of Adiponectin,FABP4,FAS and Acly detected by RT-qPCR.RESULTS Tectorigenin concentration of 60μmol/L or lower levels left no impact upon the cell viability(P>0.05).Compared with the model group with induced differentiation,the groups intervened with tectorigenin administration displayed decreased formation of lipid droplets;lower levels of TG and NEFA(P<0.01);decreased protein expressions of C/EBPα,PPARγ,perilipin-1 and ADFP(P<0.01);increased protein expressions of AMPKαand p-AMPKα(P<0.01);and decreased mRNA expressions of Adiponectin,FABP4,FAS and Acly(P<0.01).CONCLUSION Tectorigenin inhibits preadipocyte differentiation into mature adipocytes,which may be related to its efficacy in the regulation of C/EBPα,PPARγ,AMPKαand p-AMPKα.

关 键 词：鸢尾黄素 脂肪生成 3T3-L1细胞 成脂分化 

分 类 号：R285.5[医药卫生—中药学]