芥蓝游离小孢子培养技术优化及其在育种中的应用  

Optimization and Application in Breeding of Isolated Microspore Culture Technology in Chinese Kale

作　　者：张德双[1] 赵泓[1] 辛晓云 张凤兰[1] 余阳俊[1] 于拴仓[1] 苏同兵 赵岫云[1] 汪维红[1] 李佩荣 王姣[1] 卢桂香[1] ZHANG Deshuang;ZHAO Hong;XIN Xiaoyun;ZHANG Fenglan;YU Yangjun;YU Shuancang;SUTongbing;ZHAO Xiuyun;WANG Weihong;LI Peirong;WANG Jiao;LU Guixiang(Beijing Vegetable Research Center,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100097,China)

机构地区：[1]北京市农林科学院蔬菜研究所,北京100097

出　　处：《中国蔬菜》2024年第5期75-83,共9页China Vegetables

基　　金：“十四五”国家重点研发计划项目(2022YFD1200805);国家大宗蔬菜产业技术体系项目(CARS-23-A-05);北京蔬菜种质库资源繁育、优选与创新项目(KJCX20230126);北京市设施蔬菜创新团队项目(BAIC01-2023)。

摘　　要：以16个芥蓝品种(F1)为供体材料,比较分析了优化花蕾的消毒、小孢子的游离释放以及过滤等环节对芥蓝游离小孢子培养效果的影响。结果表明,16份参试芥蓝供体材料都具有胚胎发生能力,并全部获得了不同类型的再生植株,实现了较大规模、批量地获得芥蓝双单倍体(DH)。经田间综合评价筛选,应用DH系育成了不同熟性的芥蓝一代杂种20M4芥蓝、21M2芥蓝和22M11芥蓝。Taking 16 Chinese kale F1 hybrids as donor material,this paper compared and analyzed the effects of optimized buds disinfection,isolated microspore release and filtering on culture of Chinese kale free microspore.The results indicated that the 16 tested Chinese kale donor materials all had embryogenesis capability,and obtained regenerated plants of different types.Thus,a larger-scale and large quantities doubled haploid(DH)of Chinese kale were obtained.Through comprehensive evaluation and screening in the field,these DH lines by isolated microspore culture technology of Chinese kale were used in breeding,and 3 new Chinese kale F1 hybrids‘20M4 Jielan’‘21M2 Jielan’‘22M11 Jielan’with different maturity were bred.

关 键 词：芥蓝 游离小孢子 单核靠边期 双单倍体 

分 类 号：S635.9[农业科学—蔬菜学]