辣椒疫病生防菌筛选及其防效  

Screening and Prevention Effect Study of Biocontrol Bacteria of Pepper Blight

作　　者：赵沛 邢敏[1] 康萍芝[1] ZHAO Pei;XING Min;KANG Pingzhi(Institute of Plant Protection,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,Ningxia,China)

机构地区：[1]宁夏农林科学院植物保护研究所,宁夏银川750002

出　　处：《中国蔬菜》2024年第5期84-90,共7页China Vegetables

基　　金：宁夏回族自治区农业科技自主创新资金农业高质量发展和生态保护科技创新示范项目(NGSB-2021-10-01)。

摘　　要：为筛选可用于防治辣椒疫病的生防菌株,从辣椒植株内分离得到1株内生细菌菌株S39,通过平板对峙法和牛津杯法测定了菌株S39及其代谢产物对辣椒疫霉菌的抑制效果;并对其抑菌谱、防病促生效果、对根际土壤微生物种群的影响及其生防因子进行分析。结果表明:菌株S39对辣椒疫霉菌平板对峙抑菌率为83.00%,其次生代谢产物抑菌率达到75.17%;且菌株S39对多种病原菌都有抑制作用,抑菌率为63.80%~86.33%。温室盆栽试验结果表明其对辣椒疫病的防效可达75.87%;且对辣椒植株具有显著促生效果,根长增长率为21.05%。同时,菌株S39能显著增加植株根际土壤中细菌及放线菌的数量;能产生蛋白酶、淀粉酶、纤维素酶、葡聚糖酶等抗菌物质,作为生防制剂具有良好的应用前景。In order to screen the biocontrol strains which can be used to control capsicum blight,in this study,an endophytic bacterium strain S39 was isolated from a pepper plant,and the inhibitory effect of strain S39 and its metabolites on Phytophthora capsicum was determined by plate confrontation method and Oxford cup method.The antibacterial profile,disease prevention and growth promotion effect of strain S39,the effect on rhizosphere soil microbial population and its biocontrol factors were analyzed.The results showed that the antibacterial rate of strain S39 against Phytophthora capsicum flat plate was 83.00%,and the antibacterial rate of secondary metabolites reached 75.17%.Moreover,strain S39 had inhibitory effect on a variety of pathogenic bacteria,and the bacteriostatic rate reached 63.80%-86.33%.The greenhouse potting test showed that its prevention effect against pepper blight could reach 75.87%.It had a significant growth promotion effect on pepper plants,and the root growth rate was 21.05%.At the same time,it can increase the number of bacteria and actinomycetes in the rhizosphere soil.It can produce antibacterial substances such as protease,amylase,cellulase,dextran enzyme,etc.,and has a good application prospect as a biocontrol preparation.

关 键 词：辣椒疫病 内生细菌 生物防治 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S436.418.1[农业科学—植物保护]