淹水胁迫下中国南瓜转录组及差异表达基因分析  

Transcriptome and Differential Gene Analysis of Pumpkin Material under Waterlogging Stress

作　　者：刘珍宇 王鹏伟 郭林鑫 陈碧华 孙丽 李新峥 刘振威 LIU Zhenyu;WANG Pengwei;GUO Linxin;CHEN Bihua;SUN Li;LI Xinzheng;LIU Zhenwei(College of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang 453003,Henan,China;Henan Provincial Research Center for Horticultural Plant Resource Utilization and Germplasm Innovation Engineering,Xinxiang 453003,Henan,China)

机构地区：[1]河南科技学院园艺园林学院,河南新乡453003 [2]河南省园艺植物资源利用与种质创新工程研究中心,河南新乡453003

出　　处：《中国蔬菜》2024年第5期105-114,共10页China Vegetables

基　　金：河南省科技攻关项目(242102110302)。

摘　　要：近年来,受全球气候变化的影响,洪涝灾害频繁发生,已严重影响我国南瓜生产。为研究南瓜耐涝机理,以耐涝性强和淹水敏感的2份中国南瓜为试材,采用双套盆法进行淹水胁迫,利用转录组测序及实时荧光定量PCR进行差异表达基因分析。结果表明:淹水胁迫后,耐涝型南瓜材料013-2叶片氧化酶基因相对表达量整体上调,均高于淹水敏感型南瓜材料367-2;013-2和367-2叶片鉴定出3231个差异表达基因,其中上调表达基因1778个,下调表达基因1453个。GO富集发现差异表达基因显著富集在光合作用、乙烯反应、磷酸信号转导系统、细胞对乙烯刺激的反应、乙烯激活信号通路等生物过程中;KEGG注释发现有947个差异表达基因被注释到121条代谢通路中,包括光合作用-天线蛋白、植物激素信号转导、MAPK信号通路、植物-病原体相互作用、苯丙烷类生物合成等。KEGG GSEA发现植物激素信号转导、昼夜节律、MAPK信号通路、植物-病原互作等基因集得最高分。研究结果可为进一步探索与南瓜耐涝相关的基因奠定基础。In recent years,affected by global climate change,flood disaster happened frequently,has seriously affected the north pumpkin production.To study the mechanism of pumpkin waterlogging tolerance,in this experiment,two pumpkin materials with strong waterlogging tolerance and waterlogging sensitivity were selected as the research objects.Using the double pot method for flooding stress,the expression level of antioxidant enzymes was determined by qRT-PCR,transcriptional sequencing was performed by RNA-seq,and the differentially expressed genes were screened and analyzed for functional annotation.The results were as follows:after flooding stress,the relative expression level of oxidase gene in leaves of waterlogging tolerant material‘013-2’was generally up-regulated,and all of them were higher than that of waterlogging sensitive material‘367-2’.Transcriptome sequencing showed that 3231 differentially expressed genes were identified in‘013-2’and‘367-2’leaves,including 1778 up-regulated genes and 1453 down-regulated genes.GO enrichment showed that differentially expressed genes were significantly enriched in photosynthesis,ethylene reaction,phosphoric acid signal transduction system,cell response to ethylene stimulation,ethylene activation signal pathway and other biological processes.KEGG annotation revealed that 947 differentially expressed genes were annotated in 121 metabolic pathways,which were significantly enriched in photosynthesis-antenna protein,plant hormone signal transduction,MAPK signal pathway-plant,plant-pathogen interaction,phenylpropane biosynthesis and other metabolic pathways.KEGG GSEA found that plant hormone signal transduction,circadian rhythm-plant,MAPK signal pathway-plant,plant-pathogen interaction and other gene sets scored the highest.KEGG GSEA found that genes related to plant hormone signal transduction,circadian rhythm,MAPK signaling pathway,and plant pathogen interaction received the highest scores.This study laid a foundation for further exploration of regulatory and func

关 键 词：南瓜 淹水胁迫 实时荧光定量PCR RNA高通量测序技术 差异表达基因 

分 类 号：S642.1[农业科学—蔬菜学]