棕鞭金藻异养-光照耦合高密度培养生产岩藻黄素条件优化  

OPTIMIZATION OF CONDITIONS FOR HETEROTROPHIC-LIGHT COUPLING HIGH-DENSITY CULTIVATION OF POTERIOOCHROMONAS MALHAMENSIS FOR FUCOXANTHIN PRODUCTION

作　　者：郭羽芬 王诗洁 陈宝峰 王思 门君 乔志仙[3] 罗洪镇 金虎 GUO Yu-Fen;WANG Shi-Jie;CHEN Bao-Feng;WANG Si;MEN Jun;QIAO Zhi-Xian;LUO Hong-Zhen;JIN Hu(School of Life Science and Food Engineering,Huaiyin Institute of Technology,Huai’an 223003,China;Center for Molecular and Cellular Biology of Aquatic Organisms,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;The Analysis and Testing Center of Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China)

机构地区：[1]淮阴工学院生命科学与食品工程学院,淮安223003 [2]中国科学院水生生物研究所水生生物分子与细胞生物学研究中心,武汉430072 [3]中国科学院水生生物研究所分析测试中心,武汉430072

出　　处：《水生生物学报》2024年第6期1029-1039,共11页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(21808075);江苏高校“青蓝工程”优秀青年骨干教师项目;国家重点研发计划(2018YFD0901500)资助。

摘　　要：为探究棕鞭金藻生产岩藻黄素的综合性能,以期建立高效合成岩藻黄素的发酵工艺,研究首先对金藻发酵培养基中的碳氮比、维生素添加量进行优化。结果表明,当碳氮比为24、维生素添加量为3.75 mg/L维生素B_(1)和1.25 mg/L维生素B_(12)时,有利于金藻细胞内的岩藻黄素高效合成。此外,通过调控金藻光/暗培养模式,对其在不同条件下的细胞生长、最大光合效率和岩藻黄素积累情况进行比较研究,结果显示从种子培养到上罐发酵全过程进行光诱导后,胞内岩藻黄素含量显著提升。通过系统优化,金藻发酵后的胞内岩藻黄素含量和产量分别达到1.79 mg/g和33.6 mg/L,比优化前的发酵水平提高33.6%和31.2%。研究利用发酵优化技术强化了棕鞭金藻的岩藻黄素积累,为后续进一步进行大规模过程放大奠定了前期基础,也为其他微藻生产岩藻黄素提供了借鉴和参考。Fucoxanthin,a carotenoid pigment primarily derived from brown macroalgae and marine microalgae,stands out as one of the most abundant carotenoid,constituting over 10%of the total natural carotenoids production.Its distinctive molecular structure renders it applicable in various industries,including health care,medicine,cosmetic,and others.Previous studies have reported that Poterioochromonas malhamensis can accumulate fucoxanthin through a photoinduction strategy,achieving a relatively high level of 25.6 mg/L under inorganic nitrogen source culture conditions.Despite the promising efficiency displayed by P.malhamensis in fucoxanthin production efficiency,commercial fucoxanthin production remains a formidable challenge.Therefore,enhancing the overall performance of fucoxanthin production from P.malhamensis through process engineering strategies is crucial.In this study,in order to further improve the comprehensive performance of fucoxanthin production by P.malhamensis,the fermentation conditions including carbon-to-nitrogen (C/N) ratio of the P.malhamensis fermentation medium,and the adding concentration of vitamins into fermentation medium were firstly investigated.The results showed that a C/N ratio of 24,along with the additions of 3.75 mg/L of vitamin B1 and 1.25 mg/L of vitamin B12,fostered efficiently fucoxanthin synthesis in P.malhamensis cells.Additionally,a comparative study of P.malhamensis growth,maximum photosynthetic efficiency,and fucoxanthin accumulation under different light and dark culture modes was conducted.The results showed a significant enhancement in intracellular fucoxanthin content with continuous light induction throughout the entire culture process,from seed culture to fermentation in the stirred bioreactor.Through systematic optimization of the culture process,intracellular fucoxanthin content and yield reached higher levels of 1.79 mg/g and 33.6 mg/L,respectively.These values were 33.6%and 31.2%higher than those achieved in the non-optimized process.This study effectively enhanced the

关 键 词：岩藻黄素 马勒姆杯棕鞭金藻 异养-光照耦合培养 微藻发酵 

分 类 号：Q815[生物学—生物工程]