土家药刺老苞不同极性提取物的HPLC指纹图谱方法验证研究  

Validation of HPLC Fingerprint Method for Different Polarity Extracts of Tujia Medicine Aralia Chinensis L

作　　者：胡贵瑶 刘春艳 柴艺汇 王焕珍 晏朝操 曾凡勇 付强 牛建均 HU Guiyao;LIU Chunyan;CHAI Yihui;WANG Huanzhen;YAN Chaocao;ZENG Fanyong;FU Qiang;NIU Jianjun(Basic Medical College,Guizhou University of Traditional Chinese Medicine,Guizhou Province,Guiyang 550000,China;Tujia Medical Research Institute,Dejiang County National Hospital of Traditional Chinese Medicine,Guizhou Province,Dejiang 565200,China)

机构地区：[1]贵州中医药大学基础医学院,贵州贵阳550000 [2]德江县民族中医院土家医药研究所,贵州德江565200

出　　处：《中国中医药现代远程教育》2024年第13期150-153,共4页Chinese Medicine Modern Distance Education of China

基　　金：贵州中医药大学大学生创新创业训练计划项目【No.贵中医大创合字〔2022〕43号】;贵州省中医药管理局科研项目【No.QZYY-2021-092】;贵州中医药大学科研项目【No.2021】。

摘　　要：目的建立一种可用于不同极性刺老苞提取物的高效液相色谱(HPLC)指纹图谱方法。方法采用HPLC法对色谱条件进行优化,并进行方法学验证。结果色谱条件为Agilent C_(18)(250 mm×4.6 mm,5μm)色谱柱,乙腈(A)-0.1%磷酸(B)流动相梯度洗脱,检测波长280 nm,柱温30℃,进样量10μL。方法学验证显示,该HPLC指纹图谱法精密度、稳定性、重复性的相对标准偏差(RSD)符合要求。结论该HPLC指纹图谱法可同时应用于刺老苞不同极性提取物的检验,可初步比较各提取物之间的差异。Objective To establish a high-performance liquid chromatography(HPLC)fingerprint method that can be used for extracts of different Aralia chinensis L.Methods The HPLC method was used to optimize in the chromatographic conditions,and to validate their methodology.Results Chromatographic conditions were Agilent C_(18)(250 mm×4.6 mm,5μm),acetonitrile-0.1%phosphoric acid,gradient elution,280 nm,30℃,10μL.The RSD for precision,stability,and repeatability in methodological validation meets the re-quirements.Conclusion The HPLC fingerprint method can be simultaneously applied to extracts of different polarity from Aralia chi-nensis L,and can preliminarily compare the differences between extracts of different polarity from Aralia chinensis L.

关 键 词：土家药 刺老苞 不同极性提取物 HPLC 指纹图谱 实验研究 

分 类 号：R284.1[医药卫生—中药学]