南昌地区血小板供者HLA-A、B和HPA基因多态性分析及分型库的建立  

Analysis of HLA-A,B and HPA Gene Polymorphism of Platelet Donors in Nanchang and Establishment of Typing Library

作　　者：徐晶 舒轶 杜忻 余真强 徐晓琴[3] 周斌 魏小文 XU Jing;SHU Yi;DU Xin;YU Zhenqiang;XU Xiaoqin;ZHOU Bin;WEI Xiaowen(Nanchang Central Blood Station,Nanchang 330025;Ji'an City Center Blood Station,Ji'an 343000;Jiujiang Vocational and Technical College,Jiujiang 330499;Jiangxi Dean Huaxing Medical Testing Laboratory,Nanchang 330000,Jiangxi,China)

机构地区：[1]南昌市中心血站,江西南昌330025 [2]吉安市中心血站,江西吉安343000 [3]九江职业技术学院,江西九江330499 [4]江西迪安华星医学检测实验室,江西南昌330000

出　　处：《现代诊断与治疗》2024年第2期159-161,171,共4页Modern Diagnosis and Treatment

基　　金：2021年南昌市重点专科建设项目{项目编号洪卫体改字[2019]4号};江西省卫生厅科技计划项目(项目编号SKJP220229171)。

摘　　要：目的通过对血小板捐献者HLA-A、B和HPA 1-6,10,15,21基因位点的检测,分析基因位点分布特点,加强南昌地区血小板供者资料库的建设,保障临床血小板输注的有效性、及时性,提高临床输血疗效。方法对南昌地区800位血小板捐献者血样,采用荧光定量PCR的方法,对HLA-A、B位点、HPA 1-6,10,15,21位点进行基因分型,计算基因频率和基因型频率。结果HLA-A、B位点共检出HLA-A、HLA-B等位基因45个,其中HLA-A等位基因16个,频率较高的有A2、A11、A24;检出HLA-B等位基因29个,频率较高的有B13、15、B40、B46。HPA 1-6,10,15,21位点不配合率较高的是HPA3和HPA15,除此之外不配合率均低于10%。结论南昌地区人群与其它汉族地区HPA-HLA基因多态性类似。考虑其它汉族地区基因资料库计算结果和南昌ABO血型分布频率,资料库总人数需要达到7000人才能比较好的满足患者需求。Objectives By analyzing the distribution characteristics of gene loci HLA-A,B,and HPA 1-6,10,15,and 21 from platelet donors through detection,this paper aims to strengthen the establishment of a comprehensive platelet donor database in Nanchang,thusensuring theeffectiveness and timeliness of clinical platelet transfusion and improving the efficacy of clinical blood transfusion.Methods The blood samples of 800 platelet donors in Nanchang were genotyped for HLA-A,B loci and HPA 1-6,10,15,21 loci through fluorescence quantitative PCR,and the gene frequency and genotype frequency were calculated.Results A total of 45 HLA-A and HLA-B alleles were detected at HLA-A and B loci,of which 16 were HLA-A alleles,and A2,A11 and A24 were with higher frequency.A total of 29 HLA-B alleles were detected,and B13,15,B40 and B46 had higher frequencies.HPA3 and HPA15 had higher mismatch rate at HPA 1-6,10,15,and 21 loci,and the mismatch rate was less than 10%.Conclusions The HPA-HLA gene polymorphism in the population of Nanchang exhibits similarities to that observed in other Han ethnic regions.We consider the gene databases of other Han ethnic regions to obtain computational results and the distribution frequency of ABO blood types in Nanchang,as a database comprising 7000 individuals would be necessary to better meet the demands of patients.

关 键 词：血小板 HLA分型 HPA分型 基因频率 基因型频率 

分 类 号：R457[医药卫生—治疗学]