爪哇虫草菌JS001浸种处理对水稻生长和防御褐飞虱的影响  

Effects of seed soaking with Cordyceps javanica JS001 on rice growth and defense against Nilaparvata lugens(Hemiptera:Delphacidae)

作　　者：牛洪涛[1] 张志春[1] 赵冬晓 王娜[1] 王利华[1] 陈丹艳 郭慧芳[1] NIU Hong-Tao;ZHANG Zhi-Chun;ZHAO Dong-Xiao;WANG Na;WANG Li-Hua;CHEN Dan-Yan;GUO Hui-Fang(Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Plant Protection,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014 [2]安徽农业大学植物保护学院,合肥230036

出　　处：《昆虫学报》2024年第4期468-476,共9页Acta Entomologica Sinica

基　　金：国家重点研发计划项目(2021YFD1401100);国家自然科学基金项目(32372540);江苏省农业科技自主创新资金项目(CX(22)2002)。

摘　　要：【目的】明确新登记的真菌杀虫剂爪哇虫草菌Cordyceps javanica JS001在调控水稻生长和防御褐飞虱Nilaparvata lugens中的作用。【方法】室内分别用1×10^(4),1×106和1×10^(8)孢子/mL爪哇虫草菌JS001孢子悬浮液浸泡水稻种子48 h,以0.01%Triton X-100溶液浸种为对照,待培养8 d后,对水稻苗进行生长测定,并测定褐飞虱成虫对其的行为选择反应;通过气相色谱-串联质谱(GC-MS/MS)分析1×10^(8)孢子/mL爪哇虫草菌JS001孢子悬浮液浸种对水稻苗次生代谢化合物含量的影响。采用1×10^(7)孢子/mL爪哇虫草菌JS001孢子悬浮液和清水(对照)分别浸泡水稻种子48 h,于种子直播50 d后,调查稻田中稻飞虱和蜘蛛个体数量。【结果】室内测定结果表明,与对照相比,1×10^(6)和1×10^(8)孢子/mL爪哇虫草菌JS001孢子悬浮液浸种处理不仅显著促进水稻种子发芽、水稻苗根和茎叶的生长,而且降低褐飞虱成虫对水稻苗的选择性。化合物分析结果表明,与对照相比,1×10^(8)孢子/mL爪哇虫草菌JS001孢子悬浮液浸种处理的水稻苗中防御性化合物过氧化氢、油酸、辛烷等含量显著增加,能吸引褐飞虱的化合物2-壬酮含量显著降低。田间试验结果表明,与对照相比,1×10^(7)孢子/mL爪哇虫草菌JS001孢子悬浮液浸种显著降低稻田中稻飞虱数量,但对捕食性天敌蜘蛛个体数量无影响。【结论】爪哇虫草菌JS001浸种处理不但能促进水稻苗的生长,而且能提高水稻对褐飞虱的防御能力。田间试验进一步明确了爪哇虫草菌JS001在增强水稻防御稻飞虱危害中的作用。这些结果可为爪哇虫草菌的应用及稻飞虱生物防治策略的制定提供新途径。【Aim】To clarify the role of the newly registered fungal insecticide Cordyceps javanica JS001 in regulation of rice growth and defense against the brown planthopper,Nilaparvata lugens.【Methods】Rice seeds were soaked with different concentrations(1×10^(4),1×10^(6)and 1×10^(8)conidia/mL)of C.javanica JS001 conidial suspension for 48 h in laboratory,and those soaked with 0.01%Triton X-100 solution were used as the control.After 8 d of culture,the growth of rice seedlings was measured,and the behavior selection responses of N.lugens adults to rice seedlings were determined.Effects of seed soaking with 1×10^(8)conidia/mL of C.javanica JS001 conidial suspension on the contents of secondary metabolic compounds in rice seedlings were analyzed by GC-MS/MS.The rice seeds were soaked for 48 h in a C.javanica JS001 conidial suspension at the concentration of 1×10^(7)conidia/mL and clean water(control),respectively,and the individual numbers of rice planthoppers and spiders were investigated at 50 d after direct seeding in paddy fields.【Results】The results showed that compared to the control,seed soaking in C.javanica JS001 conidial suspension at the concentrations of 1×10^(6)and 1×10^(8)conidia/mL not only significantly promoted rice seed germination,and root and shoot growth of rice seedlings,but also reduced the selectivity of N.lugens adults to rice seedlings.The results of compound analysis showed that the contents of defensive compounds such as hydrogen peroxide,oleic acid and octane were significantly higher in rice seedlings from seeds soaked with 1×10^(8)conidia/mL of C.javanica JS001 conidial suspension than those in the control.Whereas the content of 2-nononone,a compound attracting N.lugens,was significantly lower in rice seedlings from seeds soaked with C.javanica JS001 conidial suspension than that in the control.Field trials showed that,compared with the control,seed soaking with 1×10^(7)conidia/mL of C.javanica JS001 conidial suspension significantly reduced the number of rice planthoppers

关 键 词：爪哇虫草菌 水稻 稻飞虱 选择行为 生物防治 

分 类 号：Q965.8[生物学—昆虫学]