大螟成虫在高剂量性信息素交配干扰环境中的求偶和交配行为  

Calling and mating behaviors of Sesamia inferens(Lepidoptera:Noctuidae)adults in the environment of high-dose sex pheromone for mating disruption

作　　者：桂嘉唯 王未英 姚晓明 伍绍龙 刘天波 林宇丰 赵洪 郭前爽 杜永均[1] GUI Jia-Wei;WANG Wei-Ying;YAO Xiao-Ming;WU Shao-Long;LIU Tian-Bo;LIN Yu-Feng;ZHAO Hong;GUO Qian-Shuang;DU Yong-Jun(Institute of Pesticide and Environmental Toxicology,Zhejiang University,Hangzhou 310058,China;Comprehensive Service Center of Sanjie Town,Shengzhou,Zhejiang Province,Shengzhou 312452,China;Zhejiang Station of Plant Protection Quarantine and Pesticide Administration,Hangzhou 310029,China;Hunan Tobacco Science Research Institute,Changsha 410004,China;Hunan Provincial Plant Protection and Quarantine Station,Changsha 410006,China;Shengzhou Agricultural Technology Extension Center of Zhejiang Province,Shengzhou 312400,China)

机构地区：[1]浙江大学农药与环境毒理研究所,杭州310058 [2]浙江省嵊州市三界镇事业综合服务中心,嵊州312452 [3]浙江省植保检疫与农药管理总站,杭州310029 [4]湖南省烟草科学研究所,长沙410004 [5]湖南省植保植检站,长沙410006 [6]浙江省嵊州市农业技术推广中心,嵊州312400

出　　处：《昆虫学报》2024年第4期507-516,共10页Acta Entomologica Sinica

基　　金：中国烟草总公司重大科技项目(110202201027(LS-11));浙江省重点研发计划项目(2018C02027)。

摘　　要：【目的】通过实验室研究高剂量性信息素环境中大螟Sesamia inferens的求偶和交配行为,分析比较性信息素化合物顺11-十六碳烯醛(Z 11-16∶Ald)和顺11-十六碳烯乙酸酯(Z 11-16∶Ac)抑制大螟成虫求偶和交配的差异,为田间大螟有效的交配干扰技术提供技术参数。【方法】在风洞生物测定仪中模拟性信息素高剂量环境,采用2-D行为记录和分析、行为观测、卵巢解剖、性信息素提取和GC-MS分析相结合的方法,测定了雌雄成虫的移动距离、求偶和交配率以及雌成虫性信息素滴度;采用主动释放器试验评价了合成的性信息素的田间交配干扰效果。【结果】大螟雄成虫在暗期第1个小时的移动距离大于其他时间段的,在Z 11-16∶Ac环境中大螟雄成虫移动距离占整个暗期移动距离的51.8%±9.3%,远高于对照(无性信息素释放)和Z 11-16∶Ald环境中的,而暗期不同时间雌成虫移动距离则没有显著差异。Z 11-16∶Ac和Z 11-16∶Ald环境中雌成虫产卵器伸出比例均显著低于对照环境中的。在对照环境中,1日龄雌成虫产卵器伸出比例为76.7%±14.6%,显著高于Z 11-16∶Ald环境中的(29.2%±13.1%)和Z 11-16∶Ac环境中的(26.7%±11.7%)。而各交配干扰环境中不同日龄大螟雌成虫产卵器开始伸出时间和伸出后的持续时间则无显著差异。对照环境中,雌雄成虫的交配率高达72.2%±5.6%,Z 11-16∶Ac和Z 11-16∶Ald环境中交配率分别为40.5%±3.4%和45.2%±3.1%,说明对照组交配率显著高于交配干扰组。在不同的交配干扰环境中,雌成虫性信息素Z 11-16∶Ald和16∶Ac的滴度无显著差异,Z 11-16∶Ac处理后顺11-十六碳烯醇(Z 11-16∶OH)和Z 11-16∶Ac的滴度显著降低,对照环境中的雌成虫Z 11-16∶OH和Z 11-16∶Ac的滴度分别为(3.79±2.56)和(7.29±4.49)ng,但Z 11-16∶Ac环境中仅分别为(0.80±0.59)和(1.99±0.45)ng。Z 11-16∶Ald环境中Z 11-16∶OH和Z 11-16∶Ac的滴度则与对照环境中�【Aim】Through the laboratory study on the calling and mating behaviors of the pink stem borer,Sesamia inferens,in a high-dose sex pheromone environment,the differences in inhibiting the calling and mating of S.inferens adults between the sex pheromone compounds cis-11-hexadecenal(Z 11-16∶Ald)and cis-11-hexadecenyl acetate(Z 11-16∶Ac)were analyzed and compared,to provide technical parameters for effective mating disruption technology of S.inferens in the field.【Methods】In the wind tunnel,a high-dose sex pheromone environment was simulated,and the moving distance,calling and mating rates of female and male adults,as well as sex pheromone titers of female adults of S.inferens were measured through a combination of 2-D behavioral recording and analysis,behavioral observation and ovarian dissection,as well as extraction and GC-MS analysis of sex pheromone.The efficacy of mating disruption by synthetic sex pheromone was evaluated in the field by using active pheromone aerosol dispensers.【Results】The moving distance of the male adults of S.inferens in the 1st hour of scotophase was longer than that in the other time periods.The moving distance of the male adults of S.inferens in the Z 11-16∶Ac environment accounted for 51.8%±9.3%of the moving distance during the entire scotophase,and was much longer than that in the control(no sex pheromone released)and Z 11-16∶Ald environments,however,no significant difference was found in the moving distance of female adults among different time periods of scotophase.The proportions of ovipositor extrusion of female adults in the Z 11-16∶Ac and Z 11-16∶Ald environments were significantly lower than that in the control environment.In the control environment,the proportion of ovipositor extrusion of the 1-day-old female adults was 76.7%±14.6%,which was significantly higher than those in the Z 11-16∶Ald and Z 11-16∶Ac environments[(29.2%±13.1%)and(26.7%±11.7%),respectively].However,there was no significant difference in the time and duration of ovipositor e

关 键 词：大螟 求偶行为 交配行为 性信息素 交配干扰 

分 类 号：Q968[生物学—昆虫学]