亚致死浓度阿维菌素和毒死蜱对经历寄主转换的稻纵卷叶螟生长发育和繁殖的影响  

Effects of a sublethal concentration of abamectin and chlorpyrifos on the growth,development and reproduction of Cnaphalocrocis medinalis(Lepidoptera:Crambidae)with the experience of host switch

作　　者：郭嘉雯 成义沙 杨亚军[1] 孙天奕 钱佳宁 鲁艳辉[1] 徐红星[1] 吴俨 吕仲贤[1] GUO Jia-Wen;CHENG Yi-Sha;YANG Ya-Jun;SUN Tian-Yi;QIAN Jia-Ning;LU Yan-Hui;XU Hong-Xing;WU Yan;L Zhong-Xian(State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products,Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Key Laboratory of Surveillance and Management of Invasive Alien Species,Guizhou Education Department,Department of Biology and Engineering of Environment,Guiyang University,Guiyang,550005,China;College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]浙江省农业科学院植物保护与微生物研究所,农产品质量安全危害因子与风险防控省部共建国家重点实验室,杭州310021 [2]贵阳学院生物与环境工程学院,贵州省高等学校外来入侵生物监测与防控重点实验室,贵阳550005 [3]南京农业大学植物保护学院,南京210095

出　　处：《昆虫学报》2024年第4期517-527,共11页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(32202280);国家水稻产业技术体系(CARS-01);贵州省教育厅重点实验室项目(黔教技[2023]024号);贵阳市高层次科技创新人才项目(筑科合同[2021]43-16)。

摘　　要：【目的】本研究旨在明确寄主转换经历是否会改变亚致死浓度杀虫剂对稻纵卷叶螟Cnaphalocrocis medinalis生长发育和繁殖的影响,为杀虫剂的合理使用及稻纵卷叶螟的综合治理提供理论依据。【方法】从稻纵卷叶螟水稻种群和小麦种群中收集卵,并将其分别转换寄主植物饲养至2龄,作为具有不同寄主转换经历处理的幼虫[以水稻为食的水稻种群(R-R)、以小麦为食的水稻种群(R-W)、以小麦为食的小麦种群(W-W)和以水稻为食的小麦种群(W-R)]。利用离体叶片浸渍法对这些幼虫在亚致死浓度(LC_(25))阿维菌素和毒死蜱浸渍下的水稻或小麦叶片处理后48 h,测定3-5龄幼虫生长发育及成虫繁殖能力和寿命。【结果】未经杀虫剂处理时,寄主转换显著影响稻纵卷叶螟3-5龄幼虫总历期、化蛹率和蛹重,但对水稻种群和小麦种群的影响相反。LC_(25)浓度阿维菌素和毒死蜱处理2龄幼虫,显著延长3-5龄幼虫总历期,但延长程度因寄主转换经历而异。LC_(25)浓度阿维菌素显著降低各寄主转换经历幼虫的化蛹率(R-R组除外)。LC_(25)浓度毒死蜱仅显著降低W-W组的化蛹率。在2龄幼虫暴露于LC_(25)浓度阿维菌素后W-W和W-R组的蛹重显著降低。水稻种群化蛹率和蛹重受寄主转换的影响在LC_(25)浓度阿维菌素处理后消失,而小麦种群化蛹率和蛹重受寄主转换的影响在LC_(25)浓度阿维菌素和毒死蜱处理后均消失。无论是何种寄主转换经历还是LC_(25)浓度阿维菌素和毒死蜱处理均未影响成虫羽化率、交配率和单雌产卵量。寄主转换处理只显著影响未经杀虫剂处理时水稻种群的卵孵化率,但卵孵化率均未受LC_(25)浓度阿维菌素和毒死蜱处理影响。与未经杀虫剂处理相比,LC_(25)浓度阿维菌素和毒死蜱处理均未影响雌成虫寿命,但LC_(25)浓度毒死蜱处理显著增加了W-W组雄成虫寿命。【结论】寄主转换经历会影响阿维菌【Aim】The aim of this study is to clarify whether the experience of host switch will alter the effects of sublethal concentrations of insecticides on the growth,development and reproduction of Cnaphalocrocis medinalis,so as to provide a theoretical basis for the rational use of insecticides and the integrated management of C.medinalis.【Methods】Eggs were collected from the rice and wheat populations of C.medinalis,and transferred to other host plants for feeding until the 2nd instar,as larvae with different experience of host switch[rice population feeding on rice(R-R),rice population feeding on wheat(R-W),wheat population feeding on wheat(W-W),and wheat population feeding on rice(W-R)].At 48 h after treatment of these larvae with the sublethal concentration(LC_(25))of abamectin and chlorpyrifos on rice or wheat leaves using the in vitro leaf impregnation method,the growth and development of the 3rd-5th instar larvae,the reproductive ability and longevity of adults were determined.【Results】Without insecticide treatment,host switching significantly affected the total duration of the 3rd-5th instar larvae,pupation rate,and pupal weight of C.medinalis,but had opposite effects on the rice and wheat populations.Treatments with the LC_(25)of abamectin and chlorpyrifos to the 2nd instar larvae significantly prolonged the total duration of the 3rd-5th instar larvae,but the degree of extension varied depending on the experience of host switch.LC_(25)of abamectin significantly reduced the pupation rate of larvae undergoing different host switches(except for the R-R group).LC_(25)of chlorpyrifos only significantly reduced the pupation rate of the W-W group.The pupal weight of the W-W and W-R groups significantly decreased after exposure of the 2nd instar larvae to LC_(25)of abamectin.The effects of host switching on the pupation rate and pupal weight of rice populations disappeared after treatment with LC_(25)of abamectin,while the effects of host switching on the pupation rate and pupal weight of wheat population

关 键 词：稻纵卷叶螟 寄主转换 阿维菌素 毒死蜱 生长发育 繁殖 

分 类 号：Q968[生物学—昆虫学]