尾悬吊模拟失重对小鼠肝脏及结肠DNA甲基化谱的影响  

Effect of tail suspension simulated weightlessness on DNA methylation profiles in liver and colon of mice

作　　者：卢燕[1,2] 郭明洲 李惠凯 陈倩倩 邱孝丰 令狐恩强 Lu Yan;Guo Mingzhou;Li Huikai;Chen Qianqian;Qiu Xiaofeng;Linghu Enqiang(Medical School of Chinese PLA,Beijing 100853,China;Department of Gastroenterology&Hepatology,the First Medical Center,Chinese PLA General Hospital,Beijing 100853,China;Northern Medical Branch of PLA General Hospital,Beijing 100094,China)

机构地区：[1]中国人民解放军医学院,北京100853 [2]中国人民解放军总医院第一医学中心消化内科医学部,北京100853 [3]中国人民解放军总医院京北医疗区本级门诊部,北京100094

出　　处：《航天医学与医学工程》2024年第1期47-51,共5页Space Medicine & Medical Engineering

摘　　要：目的通过全基因组甲基化捕获测序技术,在尾悬吊模拟失重条件下,筛选小鼠肝脏和结肠DNA甲基化的差异性位点和区域,探讨失重对基因甲基化的具体影响。方法实验采用6只8周龄的雄性C57BL/6J品系小鼠,随机分为尾吊组和对照组,每组3只,进行为期42 d的尾悬吊模拟失重实验。实验结束后,从肝脏和结肠组织中提取DNA,并利用全基因组甲基化捕获测序技术进行分析。结果肝脏组织的DNA分析显示,共发现7517个差异甲基化位点和997个差异甲基化区域,涉及4892个基因。结肠组织的DNA分析显示,共筛选出70340个差异甲基化位点和12004个差异甲基化区域,影响12877个基因。基因本体论(GO)、京都基因和基因组数据库(KEGG)生物路径分析结果表明,这些差异甲基化基因主要参与了蛋白质结合、细胞粘附、细胞活化以及多种代谢途径。结论本研究通过高通量测序技术成功鉴定了模拟失重条件下小鼠肝脏和结肠的差异甲基化位点和区域,这些发现有助于深入理解在太空长期驻留对生物体基因甲基化的影响,为太空飞行相关疾病的筛选、早期诊断和治疗提供新的研究思路。Objective This study uses whole-genome methylation capture sequencing technology to screen differential sites and regions of gene methylation in mouse liver and colon under simulated weightlessness conditions to reveal the specific impact of weightlessness on gene methylation.Methods Six 8-week-old male C57BL/6J mice were randomized into the tail suspension group and the control group,with 3 in each.The 3 mice in the tail suspension group recieved tail suspension for simulated weightlessness for 42 days.After the experiment,DNA was extracted from liver and colon tissue and analyzed using genome-wide methylation capture sequencing technology.Results DNA analysis of liver tissue showed that a total of 7517 differentially methylated sites and 997 differentially methylated regions were found,involving 4892 genes.DNA analysis of colon tissue revealed 70340 differentially methylated sites and 12004 differentially methylated regions,affecting 12877 genes.GO and KEGG path analysis revealed that these differentially methylated genes were mainly involved in protein binding,cell adhesion,cell activation,and various metabolic pathways.Conclusion This study successfully identified differential methylation sites and regions in mouse liver and colon under simulated weightlessness conditions through high-throughput sequencing technology.These findings help to further understand the impact of long-term space residence on biological gene methylation.It provides new research ideas for the prevention and early treatment of space flight-related diseases.

关 键 词：模拟失重 小鼠 肝脏 结肠 全基因组 甲基化位点 甲基化区域 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]