纳氏试剂对低浓度氨氮分析的影响因素及条件优化研究  

Influencing Factors and Optimization of Conditions for Analysis ofLow Concentration of Ammonia Nitrogen by Nessler s Reagent

作　　者：周敬峰 庞碧剑 杨爽 陈燕 蓝文陆 Zhou Jingfeng;Pang Bijian;Yang Shuang;Chen Yan;Lan Wenlu(Beibu Gulf Marine Ecological Environment Field Observation and Research Station of Guangxi,Marine Environmental Monitoring Centre of Guangxi,Beihai 536000,China)

机构地区：[1]广西壮族自治区海洋环境监测中心站、北部湾海洋生态环境广西野外科学观测研究站,广西北海536000

出　　处：《环境科学与管理》2024年第5期101-106,共6页Environmental Science and Management

基　　金：广西自然科学基金项目(2023GXNSFBA026253)。

摘　　要：针对部分商品化纳氏试剂在分析低浓度氨氮时灵敏度低的问题,通过研究三种商品化纳氏试剂在不同的投加量、显色时间和显色温度下,标准曲线的质量控制情况,查明关键的影响因素并确定最佳的显色条件。实验表明,氨氮标准曲线低浓度曲线点显色不充分是质量控制不达标的主要原因,而pH是主要的影响因素。通过调整纳氏试剂投加量或调节pH的方式,可显著提高低浓度曲线点吸光度,使标准曲线满足质控要求。This study aims to solve the problem that some commercial Nessler s reagents fail to meet the quality control requirements when drawing ammonia nitrogen standard curve.It analyzes the three commercial Nessler s reagents under different dosage,color development time and color development temperature,the standard curve quality control situation to identify key influencing factors and determine optimal color development conditions.Experiments show that insufficient color development at the low concentration curve point of the ammonia nitrogen standard curve is the main reason for the failure of quality control,and pH is the main influencing factor.By adjusting the input amount of Nessler s reagent or adjusting the pH,the absorbance at the low-concentration curve point can be significantly increased,so that the standard curve of commercial Nessler s reagent can meet the quality control requirements.

关 键 词：氨氮 商品化 纳氏试剂 分光光度法 质量控制 

分 类 号：X830.2[环境科学与工程—环境工程]