西藏米拉山大花红景天不同器官内生真菌与土壤真菌群落组成及多样性分析  

Analysis of the community composition and diversity of endophytic fungi and soil fungi in different organs of Rhodiola crenulata in MilaMountain,Xizang

作　　者：张俊泽 毛康珊 索南措[1] Zhang Junze;Mao Kangshan;Suo Nancuo(School of Ecology and Environment,Tibet University,Lhasa 850000 China;School of Life Sciences,Sichuan University,Chengdu 610065,China)

机构地区：[1]西藏大学生态环境学院,拉萨850000 [2]四川大学生命科学学院,成都610065

出　　处：《西藏科技》2024年第4期36-41,共6页Xizang Science And Technology

基　　金：西藏大学“高水平人才培养计划”项目(2021-GSP-S043)。

摘　　要：大花红景天Rhodiola crenulata,多年生草本植物,属濒危藏药植物具有抗氧化、抗高山缺氧、抗疲劳、保肝、降血糖、抗辐射等功效。植物内生菌生活在健康的植物组织中,能合成植物生长所需的物质,诱导寄主植物抗病,增强植物对环境胁迫的抵抗力。根际微生物作为植物生态系统的重要组成部分,在植物生长发育过程中的营养获取、胁迫耐受及物质循环过程中都有着举足轻重的作用。因此,研究大花红景天的内生真菌及根际真菌对该物种的开发与保护及青藏高原濒危藏药植物资源的开发与保护都具有重要意义。文章采用Illumina MiSeq高通量测序方法对西藏米拉山大花红景天内生(根、茎、叶)、根际、根区真菌进行ITS1区测序后分析真菌群落组成及多样性。研究结果显示,在属水平上,根中的优势属为minutisphaera,茎中的优势属为lemonniera,叶中的优势属为lemonniera,根际土优势属为helotiales,根区土优势属为helotiales。大花红景天茎中的内生真菌多样性明显高于根和叶。此研究较系统揭示了大花红景天内生真菌及根际真菌的群落特征,为筛选大花红景天的活性菌株及大花红景天与内生菌互作研究提供了参考依据。Rhodiola crenulata,a perennial herb,is an endangered Tibetan medicinal plant with antioxidant,anti-al-pine hypoxia,anti-fatigue,liver protection,hypoglycemia,anti-radiation and other effects.Plant endophytes live in healthy plant tissues,can synthesize substances needed for plant growth,induce host plant disease resistance,and en-hance plant resistance to environmental stress.As an important part of plant ecosystems,rhizosphere microorganisms play a pivotal role in nutrient acquisition,stress tolerance and material cycling in the process of plant growth and de-velopment.Therefore,the study of endophytic fungi and rhizosphere fungi of Rhodiola crenulata is of great signifi-cance for the development and protection of this species,as well as for the development and protection of endangered Tibetan medicinal plant resources on the Tibetan Plateau.In this study,Illumina MiSeq high-throughput sequencing was used to analyze the composition and diversity of fungal communities in the ITS1 region of Rhodiola crenulata en-dophytic(roots,stems,leaves),rhizosphere,and root-zone fungi in Mila Mountain,Xizang.The results showed that at the genus level,the dominant genus was minutisphaera in roots,lemonniera in stems,lemonniera in leaves,helotia-les in rhizosphere soil,and helotiales in root zone.The diversity of endophytic fungi in the stems of Rhodiola crenula-ta was significantly higher than that in roots and leaves.The study systematically revealed the community characteris-tics of the endophytic fungi and rhizosphere fungi of Rhodiola crenulata,and provided a reference for screening the active strains of Rhodiola crenulata and the interactions between Rhodiola crenulata and endophytic fungi.

关 键 词：大花红景天 内生菌 根际土 根区土 群落组成 多样性 

分 类 号：S154.3[农业科学—土壤学] S567.239[农业科学—农业基础科学]