冀北水稻田土壤细菌群落结构和多样性研究  

Bacterial Community Structure and Diversity in Paddy Fields in North Hebei

作　　者：张莹莹 张婷 王田雄 朱旭冉 张立军 石爱丽 ZHANG Yingying;ZHANG Ting;WANG Tianxiong;ZHU Xuran;ZHANG Lijun;SHI Aili(Chengde Academy of Agricultural and Forestry Sciences,Chengde,Hebei 067000,China)

机构地区：[1]承德市农林科学院,河北承德067000

出　　处：《陕西农业科学》2024年第4期103-108,共6页Shaanxi Journal of Agricultural Sciences

基　　金：承德市科学技术局2022年承德市基础研究项目“基于G-四链体的核酸辅助检测沙门氏菌技术研究”(编号:202205B064);承德市农林科学院自主科研项目“冀北水稻种质资源评价与创制”(编号:202301A010)。

摘　　要：为探讨冀北水稻田土壤细菌群落结构和多样性,以承德地区水稻主产区(河北隆化,河北滦平)稻田土壤为试验材料,采用高通量测序技术,对水稻大田土壤细菌群落结构和多样性进行研究。结果表明:不同地区水稻田土壤微生物多样性存在差异,其中样本ZYY.CKD9中微生物多样性最高且有益菌较多,主要有硫杆菌属(Thiobacillus)、拟衣藻属(Dechloromonas)和地杆菌属(Geobacter);但也存在共有细菌菌群,如Kaistobacter、溶杆菌属(Lysobacter)、乳酸杆菌属(Lactobacillus)、Candidatus_Solibacter、硫杆菌属(Thiobacillus)、红长命菌属(Rubrivivax)、拟衣藻属(Dechloromonas)、Anaeromyxobacter、硝化螺旋菌属(Nitrospira)等;PCA分析结果显示,土壤细菌群落结构空间相关性不明显。这为冀北水稻田土壤修复、病害防治及功能菌肥开发,促进水稻高产栽培提供依据。To explore structure and diversity of soil bacterial communities in paddy fields in North Hebei,soil samples were collected from 10 main producing areas in Longhua and Luanping in Hebei.High-throughput sequencing technology was used to investigate the community structure and diversity of soil bacteria in paddy fields.The results showed that ZYY.CKD9 had the highest microbial diversity and a large number of beneficial bacteria,including Thiobacillus,Dechromonas,and Geobacter;However,there were also common bacterial communities such as Kaistobacter,Lysobacter,Lactobacillus,Candidatus_Solibacter,Thiobacillus,Rubrivivax,Dechloromonas,Anaeromyxobacter,Nitrospira;The PCA analysis results showed that the spatial correlation of soil bacterial community structure was not significant.This study provides a basis for soil restoration,disease prevention and control,and the development of functional bacterial fertilizers to promote high-yield cultivation of rice in North Hebei.

关 键 词：水稻 根际土壤 高通量测序 细菌 

分 类 号：S154.3[农业科学—土壤学] S156.99[农业科学—农业基础科学]