健脾宁神颗粒对抽动障碍小鼠脑组织单胺类神经递质的影响  

Effects of Jianpi Ningshen Granule(健脾宁神颗粒)on Monoamine Neurotransmitter in Brain of Mice with Tic Disorder

作　　者：梁雪[1] 李会影[1] 高佳 于映霞 LIANG Xue;LI Huiying;GAO Jia;YU Yingxia(Jilin Academy of Chinese Medicine Sciences,Changchun,Jilin 130021)

机构地区：[1]吉林省中医药科学院,吉林长春130021

出　　处：《中国中医药科技》2024年第3期389-391,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：吉林省科技发展计划项目(02k2021008)。

摘　　要：目的:研究健脾宁神颗粒对抽动障碍小鼠脑组织单胺类神经递质的影响,以探讨健脾宁神颗粒可能的作用机制。方法:60只雄性ICR小鼠,除10只作为正常对照组外,其余各组均建立抽动障碍模型。造模成功后,将其随机分为模型对照组,健脾宁神颗粒高、中、低剂量组,硫必利阳性对照组;连续给药4周后,酶联免疫法(ELISA)检测脑组织多巴胺(DA)、5-羟色胺(5-HT)、去甲肾上腺素(NE)含量。结果:与正常对照组比较,模型对照组小鼠脑组织5-HT含量明显增加(P<0.05),DA、NE含量明显减少(P<0.05,P<0.01)。与模型对照组比较,健脾宁神颗粒高剂量组小鼠脑组织5-HT含量明显减少(P<0.05),DA、NE含量明显增加(P<0.05)。结论:调节脑组织单胺类神经递质DA、5-HT、NE水平是健脾宁神颗粒治疗抽动障碍的可能的作用机制之一。Objective:To study the effect of Jianpi Ningshen Granule on the brain monoamine neurotransmitter in mice with tic disorder,and to explore the possible mechanism of Jianpi Ningshen Granule.Methods:60 male ICR mice,except 10 as the normal control group,other groups were established tic disorder model.After successful modeling,the rats were randomly divided into model control group,high,middle and low dose groups of Jianpi Ningshen Granule,and positive control group of Tiapride,the levels of dopamine(DA),5-hydroxytryptamine(5-HT)and noradrenaline(NE)were measured by enzyme-linked immunosorbent assay(ELISA).Results:Compared with the normal control group,the content of 5-HT in the brain of the model control group increased significantly(P<0.05),while the content of DA and NE decreased significantly(P<0.05,P<0.01).Compared with the model control group,the content of 5-HT in the brain tissue of the high-dose group was significantly decreased(P<0.05),while the contents of DA and NE were significantly increased(P<0.05).Conclusion:To regulate brain monoamine eurotransmitter DA,5-HT and NE levels was one of the mechanism that Jianpi Ningshen Granule on tic disorder.

关 键 词：健脾宁神颗粒 抽动障碍 脑 单胺类神经递质 小鼠 

分 类 号：R285.5[医药卫生—中药学]