夜合安神丸镇静催眠作用的实验研究  

Experimental Study on Sedative-Hypnotic Effect of Yehe Anshen Pills(夜合安神丸)

作　　者：吴迪[1] 王清[1] 李伟[1] 张殿文[1] WU Di;WANG Qing;LI Wei;ZHANG Dianwen(Jilin Academy of Chinese Medicine Sciences,Changchun,Jilin 130012)

机构地区：[1]吉林省中医药科学院,吉林长春130012

出　　处：《中国中医药科技》2024年第3期401-404,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：吉林省中医药科技项目(2022072)。

摘　　要：目的:观察夜合安神丸的镇静催眠作用。方法:50只小鼠,随机分为5组,空白组,阳性对照组(安神补脑液5.2 mL/kg),夜合安神丸低、中、高剂量(3.2、1.6、0.8 g/kg)组,每日灌胃给药1次,连续7 d。采用小鼠自主活动、协同戊巴比妥钠睡眠实验及协同水合氯醛睡眠实验观察对小鼠直接睡眠的影响,测定并计算戊巴比妥钠诱导的小鼠睡眠时间,阈下剂量的睡眠百分率及水合氯醛阈下剂量的睡眠百分率。结果:夜合安神丸对小鼠自主活动有明显的抑制作用,可增加小鼠协同戊巴比妥钠和水合氯醛阈下剂量睡眠百分率,缩短小鼠协同戊巴比妥钠阈上剂量入睡潜伏期,延长小鼠协同戊巴比妥钠阈上剂量睡眠时间。结论:夜合安神丸具有较好的镇静催眠作用。Objective:To observe the sedative-hypnotic effect of Yehe Anshen Pills.Methods:50 mice were randomly divided into five groups:blank group,positive control group(Anshen Bunao Liquid 5.2 mL/kg),and Yahe Anshen Pill low-dose,medium-dose and high-dose(3.2,1.6,0.8 g/kg)groups,which were administrated once a day for 7 consecutive days.Autonomic activity of mice,sleep experiment of synergistic Pentobarbital sodium and sleep experiment of synergistic Chloral hydrate were applied to observe the effect on direct sleep of mice,sleep time,sleep percentage of subthreshold dose and sleep percentage of subthreshold dose of Pentobarbital sodium were measured and calculated.Results:Yehe Anshen Pills could significantly inhibit the autonomic activity of mice,increase the percentage of sleep under threshold dose of synergistic Pentobarbital sodium and Chloral hydrate,shorten the sleep latency of synergistic Pentobarbital sodium above threshold dose,and prolong the sleep time of synergistic pentobarbital sodium above threshold dose.Conclusion:Yehe Anshen Pills have sedative-hypnotic effect.

关 键 词：夜合安神丸 安神补脑液 镇静催眠 小鼠 

分 类 号：R285.5[医药卫生—中药学]