基于聚A单链DNA模板合成金纳米簇快速检测化妆品中的氯霉素  

Rapid Detection of Chloramphenicol in Cosmetics Based on Poly Adenine(poly-A)-templated Gold Nanoclusters

作　　者：曾希珂 易姿 杨丽霞 黄小贝 赵佳 ZENG Xi-ke;YI Zi;YANG Li-xia;HUANG Xiao-bei;ZHAO Jia(Changsha Institute for Food and Drug Control,Hunan Changsha 410036;National Liquor Product Quality Supervision and Inspection Center,Hunan Changsha 410036,China)

机构地区：[1]长沙市食品药品检验所,湖南长沙410036 [2]国家酒类产品质量检验检测中心,湖南长沙410036

出　　处：《广州化工》2024年第1期88-91,共4页GuangZhou Chemical Industry

基　　金：湖南省自然科学基金科药联合基金项目(2021JJ80077);国家市场监督管理总局科技计划项目(2020MK074)。

摘　　要：以聚A单链DNA为模板合成的金纳米簇,构建了一种新的荧光生物传感方法用于检测化妆品中的氯霉素。结果表明,最优试验条件为:反应温度为25℃,反应时间为5 min,缓冲液pH值为5.5。该方法用于检测氯霉素残留的检测线性范围为0.01~80μmol/L,检出限为7.0 nmol/L。用该方法测定爽肤水、面膜、玻尿酸样品中氯霉素的含量,回收率为98.12%~108.40%,相对标准偏差为2.32%~8.82%。Based on poly adenine(Poly-A)-templated gold nanoclusters(AuNCs),a novel fluorescence sensing method has been developed to detect chloramphenicol(CAP)in cosmetics.The optimized conditions found were as follows:reaction temperature was 25℃,reaction time was 5 min,buffer pH value was 5.5.The results showed that the relative fluorescence intensity displayed a good linear relationship with CAP concentration in the range from 0.01~80μmol/L with a 7.0 nmol/L detection limit.In addition,the sensor was successfully applied to the detection of CAP in toner,mask and hyaluronic acid samples,the recoveries from 98.12%~108.40%,and the relative standard deviation was between 2.32%~8.82%.

关 键 词：金纳米簇 氯霉素 荧光检测 

分 类 号：O657.3[理学—分析化学]