基于Tb-OBBA-Hemin纳米酶测定人血清中左氧氟沙星含量  

Tb-OBBA-Hemin Nanoenzyme-based Assay Levofloxacin in Human Serum

作　　者：叶强 YE Qiang(Zigong Institute of Light Industry Design and Research,Sichuan Zigong 643000,China)

机构地区：[1]自贡市轻工业设计研究院,四川自贡643000

出　　处：《广州化工》2024年第1期135-137,共3页GuangZhou Chemical Industry

摘　　要：本文对分光光度法测定人血清中左氧氟沙星的条件进行研究,建立一种测定人血清中左氧氟沙星浓度的简单方法。通过左氧氟沙星增强Tb-OBBA-Hemin的拟过氧化物酶活性,在中性条件下,催化过氧化氢氧化TMB生成蓝绿色的ox-TMB,在保持过氧化氢、TMB、Tb-OBBA-Hemin浓度相同的情况下,652 nm处吸光度与左氧氟沙星浓度成线性关系。方法线性范围为在0.025~1.25μg/mL,测试实际样品,相对标准偏差RSD在2.31%~3.97%(n=8)之间,加标回收率在93.6%~101.9%之间。The conditions for the spectrophotometric determination of levofloxacin in human serum were investigated to establish a simple method for the determination of levofloxacin concentration in human serum.The peroxidase-like activity of Tb-OBBA-Hemin was enhanced by levofloxacin,which catalyzed the oxidation of TMB by hydrogen peroxide to produce blue-green OX-TMB under neutral conditions,and the absorbance at 652 nm was linearly related to the concentration of levofloxacin while maintaining the same concentration of hydrogen peroxide,TMB,and Tb-OBBA-Hemin.The linear range of the method was from 0.025μg/mL to 1.25μg/mL,and the relative standard deviation RSDs ranged from 2.31% to 3.97%(n=8)for the actual samples tested,and the spiked recoveries ranged from 93.6% to 101.9%.

关 键 词：Tb-OBBA-Hemin 分光光度法 测定 左氧氟沙星 

分 类 号：O655.2[理学—分析化学]